Iwanaga M, Dohmae N, Fonagy A, Takio K, Kawasaki H, Maeda S, Matsumoto S
Molecular Entomology and Baculovirology, The Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 1998 Aug;120(4):761-7. doi: 10.1016/s0305-0491(98)10076-7.
Production of the sex pheromone bombykol in the silkworm, Bombyx mori, is regulated by a neurohormone termed pheromone biosynthesis activating neuropeptide (PBAN). It has been suggested that the external signal of PBAN in this species is transmitted to the intracellular cascade reactions consisting of Ca2+/calmodulin (CaM) complex and phosphoprotein phosphatase. To demonstrate the molecular mechanisms regulated by PBAN, we attempted to characterize CaM in the pheromone gland of B. mori. By using ion-exchange and RP-HPLC, B. mori CaM was purified from the cytosolic fraction of the pheromone gland. The primary structure was deduced by composition/sequence analysis and mass spectrometric analysis of the fragment peptides obtained from enzymatic and chemical fragmentations. The amino acid sequence of B. mori CaM was identical with Drosophila CaM deduced from the CaM gene of D. melanogaster, suggesting that insects have well conserved the molecule of CaM.
家蚕（Bombyx mori）中性信息素蚕蛾醇的产生受一种名为性信息素生物合成激活神经肽（PBAN）的神经激素调控。有人提出，该物种中PBAN的外部信号会传递到由Ca2+/钙调蛋白（CaM）复合物和磷蛋白磷酸酶组成的细胞内级联反应中。为了阐明PBAN调控的分子机制，我们试图对家蚕性信息素腺中的CaM进行特性分析。通过离子交换和反相高效液相色谱法，从性信息素腺的胞质部分纯化出了家蚕CaM。通过对酶解和化学裂解得到的片段肽进行组成/序列分析和质谱分析，推导了其一级结构。家蚕CaM的氨基酸序列与从黑腹果蝇（Drosophila melanogaster）的CaM基因推导的果蝇CaM相同，这表明昆虫很好地保守了CaM分子。
