Mudgett M, Bowen D V, Kindt T J
Biomed Mass Spectrom. 1977 Jun;4(3):159-71. doi: 10.1002/bms.1200040308.
Chemical ionization mass spectrometry is used at low resolution to determine the sequences of permethylated acetyl peptides. The method has been tested with 45 peptides, between 2 and 5 residues long, including examples of all of the common amino acids except cysteine and N-terminal asparagine. The isobutane chemical ionization spectra contain three principal types of N-terminal sequence ion and one type of C-terminal sequence ion. The redundant information available from these four types of sequence ion increase the reliability of the sequence determination. In prospect, isobutane chemical ionization mass spectrometry seems to be a useful technique for peptide sequence determination, and may have advantages in some cases.
化学电离质谱法在低分辨率下用于确定全甲基化乙酰化肽的序列。该方法已用45种长度在2至5个残基之间的肽进行了测试，包括除半胱氨酸和N端天冬酰胺之外所有常见氨基酸的示例。异丁烷化学电离谱包含三种主要类型的N端序列离子和一种类型的C端序列离子。这四种类型的序列离子所提供的冗余信息提高了序列测定的可靠性。展望未来，异丁烷化学电离质谱法似乎是一种用于肽序列测定的有用技术，并且在某些情况下可能具有优势。
