Zajic J E, Guignard H, Gerson D F
Biotechnol Bioeng. 1977 Sep;19(9):1285-301. doi: 10.1002/bit.260190904.
A Corynebacterium hydrocarboclastus culture isolated in our laboratory (see, Zajic and Knettig, Developments in Industrial Microbiology, 1971, p. 87) has been shown to produce an extracellular biopolymer with emulsifying properties when grown on a mixture of linear hydrocarbons. This microorganism was found to grow well on a variety of carbohydrates and hydrocarbons. However, the best substrates were pure linear hydrocarbons and particularly, n-C12, n-C13, and n-C14. The substrates supporting good growth gave good polymer production. Maximum cell mass of 10-11 g/liter and a maximum amount of polymer of 5-6 g/liter were recorded. The polymers recovered from the different substrates were found to be complex molecules or mixtures with a protein, a lipid, and a carbohydrate moiety. All the polymers are surface active and have two critical micelle concentrations.
在我们实验室分离出的一种解烃棒状杆菌培养物（见扎伊克和克内蒂格所著《工业微生物学进展》，1971年，第87页）已表明，当它在线性烃混合物上生长时会产生一种具有乳化特性的细胞外生物聚合物。已发现这种微生物能在多种碳水化合物和烃类上良好生长。然而，最佳底物是纯线性烃，尤其是正十二烷、正十三烷和正十四烷。支持良好生长的底物能产生良好的聚合物。记录到的最大细胞质量为10 - 11克/升，聚合物的最大量为5 - 6克/升。从不同底物中回收的聚合物被发现是具有蛋白质、脂质和碳水化合物部分的复杂分子或混合物。所有聚合物都具有表面活性，且有两个临界胶束浓度。
