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[大豆重复DNA的克隆、测序及特性分析]

[Cloning, sequencing and characterizing repetitive DNA of Glycine max].

作者信息

Hui D, Liu F, Chen S, Wang J, Yan Y, Miao Y

机构信息

Institute of Genetics Academia Sinica, Beijing.

出版信息

Yi Chuan Xue Bao. 1995;22(6):455-62.

PMID:8900841
Abstract

One satellite DNA family, cloned from Glycine max, was sequenced and chracterized. The unit of the repetitive sequences was 91bp. They concentrated on the arms of chromosomes M2 and M11, and spreaded on whole chromosomes Sm7 and M12. Southern blot analysis with 18 acessions, belonged to subgenus Glycine and subgenus Soj a of genus Glycine, showed that the repetitive sequences were specific to subgenus Soj a. It was subgenus specific repetitive sequence. This result, from another aspact, supported the idea that the three species in subgenus Soj a should be considered as one species.

摘要

从大豆中克隆出一个卫星DNA家族,并对其进行了测序和表征。重复序列单元为91bp。它们集中在M2和M11染色体的臂上,并分布在整个Sm7和M12染色体上。对属于大豆属大豆亚属和菽亚属的18个种质进行Southern杂交分析,结果表明这些重复序列是菽亚属特有的。它是亚属特异性重复序列。这一结果从另一个方面支持了将菽亚属中的三个物种视为一个物种的观点。

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