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[盘羊高度重复DNA的分离与分析]

[The isolation and analysis of the highly repetitive DNA from the argali].

作者信息

Fedorova E V, Rogozin I B, Ptitsyn A A, Cheriaukene O V, Kaftanovskaia E M

出版信息

Tsitol Genet. 1998 Sep-Oct;32(5):67-74.

PMID:9879117
Abstract

The repeated DNA sequence of wild ram (Ovis ammon) of 800 bp has been cloned. The blot-hybridization, in situ-hybridization, sequencing and computer analysis were used for the sequence analysis. It was shown that the cloned DNA is from 1.714 gm/cm3 repeated satellite DNA family. Fourteen highly homologous sequences were revealed in the nucleotide sequence databases. An analysis of their alignment revealed presence of two subfamilies (A and B). Average divergence of subfamily A. sequences (including the wild ram repeated sequence) from consensus is about 1%.

摘要

已克隆出野生绵羊(盘羊)800 bp的重复DNA序列。采用印迹杂交、原位杂交、测序及计算机分析进行序列分析。结果表明,克隆的DNA来自密度为1.714 gm/cm3的重复卫星DNA家族。在核苷酸序列数据库中发现了14个高度同源的序列。对它们的比对分析显示存在两个亚家族(A和B)。亚家族A序列(包括野生绵羊重复序列)与共有序列的平均差异约为1%。

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