Lu B, Westerlund D
Analytical Pharmaceutical Chemistry, Uppsala University Biomedical Centre, Sweden.
Electrophoresis. 1996 Feb;17(2):325-32. doi: 10.1002/elps.1150170207.
A rapid and sensitive method for the analysis of sugars was developed by capillary zone electrophoresis (CZE) with indirect UV detection. A mixture of nine mono- and oligosaccharides was separated in about 20 minutes on a fused silica capillary in a highly alkaline (pH > 12) background electrolyte (BGE). With tryptophan as the marker detected at 280 nm the femtomole level detection of sugars was possible. The system was optimized by studies on the buffer pH, the marker concentration, as well as the inner diameter of the capillary. The indirect detection of sugars was also performed with a cationic marker, N-benzylcinchonidium chloride (BCDC). However, the detection sensitivity was much lower in comparison to the tryptophan marker system.
建立了一种快速灵敏的糖分析方法，采用毛细管区带电泳（CZE）结合间接紫外检测。在高度碱性（pH>12）的背景电解质（BGE）中，在熔融石英毛细管上，9种单糖和寡糖的混合物在约20分钟内分离。以色氨酸为标记物，在280nm处检测，可实现糖的飞摩尔水平检测。通过研究缓冲液pH值、标记物浓度以及毛细管内径对系统进行了优化。还使用阳离子标记物氯化N-苄基辛可宁鎓（BCDC）对糖进行间接检测。然而，与色氨酸标记物系统相比，检测灵敏度要低得多。
