Andersen Keld E, Bjergegaard Charlotte, Møller Peter, Sørensen Jens C, Sørensen Hilmer
Chemistry Department, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
J Agric Food Chem. 2003 Oct 22;51(22):6391-7. doi: 10.1021/jf030328m.
A rapid, easy, and reproducible capillary electrophoresis method for determination of raffinose family oligosaccharides (alpha-galactosides) was developed. Sucrose, raffinose, stachyose, verbascose, and ajugose were determined with indirect UV detection at moderate alkaline pH 9.2, using pyridine-2,6-dicarboxylic acid as background electrolyte in a sodium tetraborate buffer with added cetyltrimethylammonium bromide. The separation efficiency measured by the number of theoretical plates (N) ranged from 1.4 x 10(5) to 2.3 x 10(5). The precision of the method, measured by the relative standard deviation (RSD), was less than 0.53% for the migration times and better than 3.4% for normalized areas (NA), considering all sugars except verbascose (RSD(NA) = 11.8%). Detection limits were about 110 microg/mL, corresponding to 150-320 microM. Relative response factors (RRF) were calculated on the basis of linearity studies and used for quantification of alpha-galactosides in a lupine sample (Lupinus angustifolius).
建立了一种快速、简便且可重复的毛细管电泳法，用于测定棉子糖家族低聚糖（α-半乳糖苷）。在pH 9.2的中等碱性条件下，以吡啶-2,6-二羧酸为背景电解质，在添加了十六烷基三甲基溴化铵的硼酸钠缓冲液中，通过间接紫外检测法测定蔗糖、棉子糖、水苏糖、毛蕊花糖和藿香糖。以理论塔板数（N）衡量的分离效率范围为1.4×10⁵至2.3×10⁵。该方法的精密度，以相对标准偏差（RSD）衡量，迁移时间的RSD小于0.53%，归一化面积（NA）的RSD优于3.4%（除毛蕊花糖外，所有糖类的RSD(NA)=11.8%）。检测限约为110μg/mL，相当于150 - 320μM。基于线性研究计算了相对响应因子（RRF），并用于定量羽扇豆样品（窄叶羽扇豆）中的α-半乳糖苷。
