Patterson S, Helbert M, English N R, Pinching A J, Knight S C
Imperial College School of Medicine at Northwick Park Institute for Medical Research, Harrow, Middlesex.
Res Virol. 1996 Mar-Jun;147(2-3):109-14. doi: 10.1016/0923-2516(96)80224-x.
In this pilot study, the numbers of dendritic cells (DC) in peripheral blood of AIDS patients and the level of infection with HIV1 were determined before and after AZT treatment. Mononuclear cells were cultured overnight and DC were identified by their lack of labelling with antibodies specific for T, B and natural killer (NK) cells and monocytes and by their high level of staining with antibodies for MHC class II molecules. Although the numbers of DC identified by this method were lower than those identified morphologically in earlier studies (Macatonia et al., 1990), the numbers in three untreated AIDS patients were below the range seen in normals. There was also a marked rise in DC number in patients given AZT therapy. In two patients, there was a significant provirus load in the DCs which was decreased two to three weeks after the commencement of AZT therapy. The studies suggest that DC numbers and their infection levels may be markers of disease in HIV infection.
在这项初步研究中，测定了艾滋病患者外周血中树突状细胞（DC）的数量以及AZT治疗前后HIV1的感染水平。单核细胞培养过夜，通过缺乏针对T、B和自然杀伤（NK）细胞以及单核细胞的特异性抗体标记，以及通过针对MHC II类分子的抗体进行高染色水平来鉴定DC。尽管用这种方法鉴定出的DC数量低于早期研究（Macatonia等人，1990年）中通过形态学鉴定出的数量，但三名未经治疗的艾滋病患者的DC数量低于正常范围。接受AZT治疗的患者的DC数量也有显著增加。在两名患者中，DC中有明显的前病毒载量，在AZT治疗开始后两到三周降低。这些研究表明，DC数量及其感染水平可能是HIV感染中疾病的标志物。
