Engelke D R, Pagán-Ramos E, Tranguch A J
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0606, USA.
Mol Biol Rep. 1995;22(2-3):157-60. doi: 10.1007/BF00988722.
Eukaryotic ribonuclease P (RNase P) enzymes require both RNA and protein subunits for activity in vivo and in vitro. We have undertaken an analysis of the complex RNA subunit of the nuclear holoenzyme in an effort to understand its structure and its similarities to and differences from the bacterial ribozymes. Phylogenetic analysis, structure-sensitive RNA footprinting, and directed mutagenesis reveal conserved secondary and tertiary structures with both strong similarities to the bacterial consensus and distinctive features. The effects of mutations in the most highly conserved positions are being used to dissect the functions of individual subdomains.
真核核糖核酸酶P（RNase P）在体内和体外发挥活性都需要RNA和蛋白质亚基。我们对核全酶的复杂RNA亚基进行了分析，以了解其结构以及与细菌核酶的异同。系统发育分析、结构敏感的RNA足迹分析和定向诱变揭示了保守的二级和三级结构，这些结构与细菌共有序列有很强的相似性，也有独特的特征。在最保守位置的突变效应正被用于剖析各个亚结构域的功能。
