Kikuchi Y
Department of Ecological Engineering, Toyohashi University of Technology, Japan.
Mol Biol Rep. 1995;22(2-3):171-5. doi: 10.1007/BF00988724.
Hyperprocessing is defined as a further processing of mature RNA that produces another functional RNA. Hyperprocessing occurs in Drosophila cells. In the transposon copia-related retrovirus-like particles of Drosophila, a 39-nucleotide-long fragment from the 5'region of Drosophila initiator methionine tRNA is used as the primer for copia minus-strand reverse transcription. This primer tRNA fragment is thought to be produced by cleavage within the mature tRNA sequence. We found that the catalytic RNA subunit of RNase P catalyzes this hyperprocessing in vitro and that this cleavage is dependent of the occurrence of an altered conformation of the tRNA substrate. In this review, I will summarize our work from the finding of the functional RNA fragment to the finding of a dynamic tRNA structure.
超加工被定义为对成熟RNA进行进一步加工，从而产生另一种功能性RNA。超加工发生在果蝇细胞中。在果蝇与转座子copia相关的逆转录病毒样颗粒中，来自果蝇起始甲硫氨酸tRNA 5'区域的一个39个核苷酸长的片段被用作copia负链逆转录的引物。这个引物tRNA片段被认为是由成熟tRNA序列内的切割产生的。我们发现，RNase P的催化RNA亚基在体外催化这种超加工，并且这种切割依赖于tRNA底物构象改变的发生。在这篇综述中，我将总结我们从功能性RNA片段的发现到动态tRNA结构的发现所做的工作。
