Teske E, van Heerde P
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Vet Q. 1996 Sep;18(3):112-5. doi: 10.1080/01652176.1996.9694630.
Cytological examination of biopsy samples obtained by fine-needle aspiration appears to be an accurate and reliable basis for the classification of canine non-Hodgkin's lymphoma (NHL). Intra- and inter-observer reproducibility of both the original and the updated Kiel classification were assessed blindly on two occasions by two experienced cytologists who examined biopsy specimens from 78 dogs with NHL. Analysis by kappa statistics showed moderate to good intra-observer reproducibility and poor to moderate inter-observer reproducibility for the original Kiel classification. In the updated Kiel classification both intra- and inter-observer reproducibilities were good, probably due in part to the additional information provided by the immunotyping. The consensus cytological classifications were compared with those based on histology, and the association constant (Cramér's V) was 0.65 for the original Kiel classification and 0.70 for the updated Kiel classification. The results suggest that the updated Kiel classification in particular is suited to cytological classification of canine NHL.
通过细针穿刺获取的活检样本的细胞学检查似乎是犬非霍奇金淋巴瘤（NHL）分类的准确且可靠的基础。两位经验丰富的细胞学家两次对来自78只患有NHL的犬的活检标本进行盲法评估，以评价原始和更新后的基尔分类法在观察者内和观察者间的可重复性。kappa统计分析显示，原始基尔分类法的观察者内可重复性为中度至良好，观察者间可重复性为差至中度。在更新后的基尔分类法中，观察者内和观察者间的可重复性均良好，这可能部分归因于免疫分型提供的额外信息。将一致的细胞学分类与基于组织学的分类进行比较，原始基尔分类法的关联常数（克莱默V系数）为0.65，更新后的基尔分类法为0.70。结果表明，特别是更新后的基尔分类法适用于犬NHL的细胞学分类。
