Kimbi H K, Fagbenro-Beyioku A F
Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Nigeria.
East Afr Med J. 1995 Nov;72(11):742-3.
In an attempt to induce chloroquine resistance in Plasmodium yoelii nigeriensis by the method of drug pressure, it was found that the mice continued to be infected until the fourth passage when they became refractory or immune to the infection. When the immune mice were challenged at four weekly intervals, they remained refractory to the infection until week 12 when one of them became infected. However, by the sixteenth week, the rest of the mice became susceptible to the infection. Four of the mice which received a booster dose of the parasite which had been exposed to chloroquine in three passages, that is, chloroquine-attenuated at the sixteenth week, did not pick up a challenge infection two weeks later while the controls did.
为了通过药物压力法诱导约氏疟原虫尼日尔株产生氯喹抗性，发现小鼠在第四次传代前一直受到感染，此后它们对感染变得难治或免疫。当对免疫小鼠每隔四周进行一次攻击时，它们在第12周之前对感染仍具抗性，直到其中一只小鼠被感染。然而，到第16周时，其余小鼠对感染变得易感。四只接受了在三次传代中接触过氯喹的寄生虫加强剂量的小鼠，即在第16周时经氯喹减毒的寄生虫，在两周后未受到攻击感染，而对照组小鼠则受到了感染。
