Kimbi H K, Fagbenro-Beyioku A F
Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Nigeria.
East Afr Med J. 1996 Oct;73(10):634-5.
Chloroquine resistance was induced in Plasmodium yoelii nigeriensis by the method of drug pressure. Twenty five albino mice were used at each passage and each of them was inoculated intra-peritoneally with approximately 10(5) erythrocytes infected with the parasite. The first fifteen mice divided into three groups of five mice each were treated with different sub-curative doses of chloroquine which usually cleared the infection for a few days before recrudescence. Inocula for passages to the next set of mice was made from the recrudesced infection. The sub-curative dose was progressively increased as rapidly as the build-up of parasitaemia permitted. The last ten mice divided into two groups of five mice each were used in the 'two percent test' to assess the level of resistance in the parasite. Full resistance to the maximum tolerated dose of chloroquine to the host mouse (80 mg/kg body weight) was reached at passage 20.
通过药物压力法在约氏疟原虫尼氏亚种中诱导氯喹抗性。每次传代使用25只白化小鼠，每只小鼠经腹腔接种约10(5)个感染该寄生虫的红细胞。将最初的15只小鼠分成三组，每组5只，用不同的亚治疗剂量氯喹进行治疗，这些剂量通常能在感染复发前清除感染数天。用于传代至下一组小鼠的接种物取自复发的感染。随着寄生虫血症的积累允许，亚治疗剂量逐渐快速增加。最后10只小鼠分成两组，每组5只，用于“2%试验”以评估寄生虫的抗性水平。在第20代时达到了对宿主小鼠最大耐受剂量氯喹（80毫克/千克体重）的完全抗性。
