通过聚合酶链反应（PCR）检测石蜡包埋宫颈癌中人类乳头瘤病毒DNA的几种通用引物对和类型特异性引物对的综合研究。
Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas.
作者信息
Baay M F, Quint W G, Koudstaal J, Hollema H, Duk J M, Burger M P, Stolz E, Herbrink P
机构信息
Department of Dermatovenereology, Erasmus University, University Hospital, Rotterdam, The Netherlands.
出版信息
J Clin Microbiol. 1996 Mar;34(3):745-7. doi: 10.1128/jcm.34.3.745-747.1996.
Abstract
We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.
摘要
我们比较了三种通用引物对在检测甲醛固定石蜡包埋癌组织中人类乳头瘤病毒（HPV）DNA的效能。使用这些引物对会导致HPV流行率被低估（GP5/6，61.1%；CPI/IIG，57.4%；MY09/11，46.9%；综合，72.8%）。每个引物对的效能似乎与所产生扩增子的长度呈负相关。通过使用新开发的型特异性引物对（扩增子长度约100 bp），发现HPV DNA检测率有所提高（87.6%）。
相似文献
1
Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas.通过聚合酶链反应（PCR）检测石蜡包埋宫颈癌中人类乳头瘤病毒DNA的几种通用引物对和类型特异性引物对的综合研究。
J Clin Microbiol. 1996 Mar;34(3):745-7. doi: 10.1128/jcm.34.3.745-747.1996.
2
Use of multiple PCR primer sets for optimal detection of human papillomavirus.使用多种聚合酶链反应引物组以实现人乳头瘤病毒的最佳检测。
J Clin Microbiol. 1996 Sep;34(9):2095-100. doi: 10.1128/jcm.34.9.2095-2100.1996.
3
Evaluation of human papillomavirus-consensus primers for HPV detection by the polymerase chain reaction.用于聚合酶链反应检测人乳头瘤病毒的人乳头瘤病毒通用引物的评估
Mol Cell Probes. 1999 Feb;13(1):9-21. doi: 10.1006/mcpr.1998.0203.
4
Human papillomavirus deoxyribonucleic acid detection in mildly or moderately dysplastic smears: a possible method for selecting patients for colposcopy.轻度或中度发育异常涂片中人乳头瘤病毒脱氧核糖核酸检测：一种选择阴道镜检查患者的可能方法。
Am J Obstet Gynecol. 1997 Sep;177(3):548-53. doi: 10.1016/s0002-9378(97)70144-3.
5
Comparative evaluation of the GP5+/6+, MY09/11 and PGMY09/11 primer sets for HPV detection by PCR in oral squamous cell carcinomas.通过聚合酶链反应（PCR）检测口腔鳞状细胞癌中HPV的GP5+/6+、MY09/11和PGMY09/11引物组的比较评估
Exp Mol Pathol. 2016 Feb;100(1):13-6. doi: 10.1016/j.yexmp.2015.11.024. Epub 2015 Nov 24.
6
Establishment of a molecular diagnostic system for detecting human papillomavirus in clinical samples in Sri Lanka.在斯里兰卡建立用于检测临床样本中人类乳头瘤病毒的分子诊断系统。
Southeast Asian J Trop Med Public Health. 2011 Nov;42(6):1423-30.
7
PCR detection of human papillomavirus of the mucosa: comparison between MY09/11 and GP5+/6+ primer sets.黏膜人乳头瘤病毒的聚合酶链反应检测：MY09/11与GP5+/6+引物组的比较
J Clin Virol. 2004 Aug;30(4):302-8. doi: 10.1016/j.jcv.2003.12.011.
8
Multiple HPV genotypes in cervical carcinomas: improved DNA detection and typing in archival tissues.宫颈癌中的多种人乳头瘤病毒基因型：存档组织中DNA检测与分型的改进
J Clin Virol. 2004 Apr;29(4):271-6. doi: 10.1016/S1386-6532(03)00167-7.
9
Multiple primer pairs polymerase chain reaction for the detection of human papillomavirus types.用于检测人乳头瘤病毒类型的多重引物对聚合酶链反应
J Virol Methods. 1990 Apr;28(1):59-65. doi: 10.1016/0166-0934(90)90087-v.
10
Development and clinical evaluation of a highly sensitive PCR-reverse hybridization line probe assay for detection and identification of anogenital human papillomavirus.用于检测和鉴定肛门生殖器人乳头瘤病毒的高灵敏度聚合酶链反应-反向杂交线性探针检测法的开发与临床评估
J Clin Microbiol. 1999 Aug;37(8):2508-17. doi: 10.1128/JCM.37.8.2508-2517.1999.
引用本文的文献
1
Head-to-head comparison of 7 high-sensitive human papillomavirus nucleic acid detection technologies with the SPF10 LiPA-25 system.7种高灵敏度人乳头瘤病毒核酸检测技术与SPF10 LiPA-25系统的直接比较。
J Natl Cancer Cent. 2022 Jul 3;2(3):148-154. doi: 10.1016/j.jncc.2022.06.003. eCollection 2022 Sep.
2
Decoding the Genetic and Structural Features of HPV16 E5 Oncogene in Cervical Cancer Isolates from Pakistan: A Pilot Study.解码来自巴基斯坦宫颈癌分离株中HPV16 E5癌基因的遗传和结构特征：一项试点研究。
Iran Biomed J. 2023 Aug 23;27(6):388-96. doi: 10.61186/ibj.3884.
3
HPV16 Impacts NHERF2 Expression in Oropharyngeal Cancers.人乳头瘤病毒16型影响口咽癌中埃兹蛋白、根蛋白和膜突蛋白2的表达。
Pathogens. 2023 Aug 3;12(8):1013. doi: 10.3390/pathogens12081013.
4
Human DLG1 and SCRIB Are Distinctly Regulated Independently of HPV-16 during the Progression of Oropharyngeal Squamous Cell Carcinomas: A Preliminary Analysis.口咽鳞状细胞癌进展过程中，人Dlg1和SCRIB独立于HPV-16受到不同调控：一项初步分析
Cancers (Basel). 2021 Sep 4;13(17):4461. doi: 10.3390/cancers13174461.
5
Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia.东亚、东南亚和南亚地区人乳头瘤病毒（HPV）致癌类型的最新进展
Cancers (Basel). 2021 May 30;13(11):2691. doi: 10.3390/cancers13112691.
6
Human DNA decays faster with time than viral dsDNA: an analysis on HPV16 using pathology archive samples spanning 85 years.人类 DNA 随时间的推移比病毒双链 DNA 降解得更快：使用跨越 85 年的病理学存档样本分析 HPV16。
Virol J. 2021 Mar 29;18(1):65. doi: 10.1186/s12985-021-01529-9.
7
Downregulated Expression of WWOX in Cervical Carcinoma: A Case-Control Study.WWOX在宫颈癌中的表达下调：一项病例对照研究。
Int J Mol Cell Med. 2020 Fall;9(4):273-288. doi: 10.22088/IJMCM.BUMS.9.4.273. Epub 2021 Jan 27.
8
Rational genomic optimization of DNA detection for human papillomavirus type 16 in head and neck squamous cell carcinoma.头颈部鳞状细胞癌中16型人乳头瘤病毒DNA检测的合理基因组优化
Head Neck. 2020 Apr;42(4):688-697. doi: 10.1002/hed.26041. Epub 2019 Dec 18.
9
Determinants of Acquisition and Clearance of Human Papillomavirus Infection in Previously Unexposed Young Women.未暴露于 HPV 感染的年轻女性 HPV 感染获得和清除的决定因素。
Sex Transm Dis. 2019 Oct;46(10):663-669. doi: 10.1097/OLQ.0000000000001053.
10
Mutational landscape of head and neck squamous cell carcinomas in a South Asian population.南亚人群头颈部鳞状细胞癌的突变图谱
Genet Mol Biol. 2019 Jul-Sep;4242(3):526-542. doi: 10.1590/1678-4685-GMB-2018-0005. Epub 2019 Nov 14.
本文引用的文献
1
Detection of cutaneous and genital HPV types in clinical samples by PCR using consensus primers.使用通用引物通过聚合酶链反应（PCR）检测临床样本中的皮肤和生殖器人乳头瘤病毒（HPV）类型。
J Virol Methods. 1993 May;42(2-3):265-79. doi: 10.1016/0166-0934(93)90038-s.
2
Modifications of human and viral deoxyribonucleic acid by formaldehyde fixation.甲醛固定对人类和病毒脱氧核糖核酸的修饰作用。
Lab Invest. 1994 Oct;71(4):604-11.
3
High incidence of human papillomavirus in 146 cervical carcinomas. A study using three different pairs of consensus primers, and detecting viral genomes with putative deletions.146例宫颈癌中人乳头瘤病毒的高发病率。一项使用三对不同的一致性引物并检测具有假定缺失的病毒基因组的研究。
Eur J Cancer. 1995;31A(9):1511-6. doi: 10.1016/0959-8049(95)00206-x.
4
Amplification of human papillomavirus DNA sequences by using conserved primers.使用保守引物扩增人乳头瘤病毒DNA序列。
J Clin Microbiol. 1989 Dec;27(12):2660-5. doi: 10.1128/jcm.27.12.2660-2665.1989.
5
Increased detection rate of human papillomavirus in cervical scrapes by the polymerase chain reaction as compared to modified FISH and southern-blot analysis.与改良荧光原位杂交和Southern印迹分析相比，聚合酶链反应提高了宫颈刮片中人乳头瘤病毒的检测率。
J Med Virol. 1989 Apr;27(4):329-35. doi: 10.1002/jmv.1890270413.
6
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
7
Detection and typing of human papillomavirus in archival cervical cancer specimens by DNA amplification with consensus primers.使用共有引物进行DNA扩增对存档宫颈癌标本中的人乳头瘤病毒进行检测和分型
J Natl Cancer Inst. 1990 Sep 19;82(18):1477-84. doi: 10.1093/jnci/82.18.1477.
8
General primer-mediated polymerase chain reaction permits the detection of sequenced and still unsequenced human papillomavirus genotypes in cervical scrapes and carcinomas.通用引物介导的聚合酶链反应可用于检测宫颈刮片和癌组织中已测序和未测序的人乳头瘤病毒基因型。
Int J Cancer. 1990 Apr 15;45(4):644-9. doi: 10.1002/ijc.2910450412.
9
The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes.在聚合酶链反应中使用通用引物可检测多种人类乳头瘤病毒基因型。
J Gen Virol. 1990 Jan;71 ( Pt 1):173-81. doi: 10.1099/0022-1317-71-1-173.
10
Zotero 插件