Polache A, Martin-Algarra R V, Guerri C
Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Valencia, Spain.
Alcohol Clin Exp Res. 1996 Oct;20(7):1237-42. doi: 10.1111/j.1530-0277.1996.tb01118.x.
The present study evaluates the effect of chronic alcohol intake on the intestinal transport of methionine during pregnancy. For this purpose, we have used an in vitro technique that allows measurement of the unidirectional influx of the amino acids across the brush-border membrane of the rat mid-jejunum, and the basolateral membrane enzyme Na+, K+-ATPase was also evaluated in the duodenum and jejunum. For chronic alcohol treatment, the rats were fed a liquid diet containing ethanol (36% of calories) or an isocaloric diet-(pair-fed control) for 5 weeks before and during pregnancy. Animals were killed at 21 days of gestation. Results from the kinetic analysis revealed that chronic ethanol treatment reduces the maximum transport (Jm) of methionine uptake when compared with controls. Further experiments performed in the presence and absence of sodium have shown that ethanol selectively inhibited Na+-dependent methionine transport. At the same time, this treatment significantly reduced the levels of Na+, K+-ATPase in ethanol-fed rats compared with the controls. Alterations in methionine intestinal transport in pregnant alcohol-fed rats may contribute to the ethanol-induced fetal growth abnormalities.
本研究评估孕期长期摄入酒精对蛋氨酸肠道转运的影响。为此，我们采用了一种体外技术，该技术可测量氨基酸跨大鼠空肠中段刷状缘膜的单向流入量，并且还评估了十二指肠和空肠中基底外侧膜酶钠钾ATP酶。对于长期酒精处理，在怀孕前及怀孕期间，给大鼠喂食含乙醇（占热量的36%）的液体饲料或等热量饲料（配对喂养对照），持续5周。在妊娠21天时处死动物。动力学分析结果显示，与对照组相比，长期乙醇处理降低了蛋氨酸摄取的最大转运量（Jm）。在有钠和无钠情况下进行的进一步实验表明，乙醇选择性抑制依赖钠的蛋氨酸转运。同时，与对照组相比，该处理显著降低了喂食乙醇的大鼠体内钠钾ATP酶的水平。怀孕的酒精喂养大鼠蛋氨酸肠道转运的改变可能导致乙醇诱导的胎儿生长异常。
