Terai K, Matsuo A, McGeer P L
Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, Canada.
Brain Res. 1996 Sep 30;735(1):159-68. doi: 10.1016/0006-8993(96)00310-1.
The distribution of nuclear factor-kappa B (NF-kappa B) was investigated immunohistochemically in the hippocampal formation, entorhinal cortex, middle temporal gyrus and visual cortex of Alzheimer's disease (AD) and control postmortem cases using a polyclonal antibody against the NF-kappa B p65 subunit. In AD cases, prominent staining for NF-kappa B was seen in neurons and their processes, neurofibrillary tangles and dystrophic neurites. In control cases, only weak staining of some neurons was obtained. The neuronal staining observed in AD was strongest in the hippocampal formation and entorhinal cortex, less in the middle temporal gyrus and least in the visual cortex. There was no difference between AD and control cases in the staining of glial cells and vascular walls. These results suggest that enhanced expression of neuronal NF-kappa B occurs in areas affected by AD pathology.
使用抗核因子-κB p65亚基的多克隆抗体，通过免疫组织化学方法研究了阿尔茨海默病（AD）和对照尸检病例海马结构、内嗅皮质、颞中回和视皮质中核因子-κB（NF-κB）的分布。在AD病例中，NF-κB在神经元及其突起、神经原纤维缠结和营养不良性神经突中可见显著染色。在对照病例中，仅获得一些神经元的弱阳性染色。AD中观察到的神经元染色在海马结构和内嗅皮质中最强，在颞中回中较少，在视皮质中最少。AD和对照病例在胶质细胞和血管壁染色方面没有差异。这些结果表明，神经元NF-κB的表达增强发生在受AD病理影响的区域。
