Christner P, Robinson P, Clark C C
Calcif Tissue Res. 1977 Jun 28;23(2):147-50. doi: 10.1007/BF02012780.
Human teeth were used to obtain cementum. Collagen could not be significantly solubilized from the cementum by salt and acetic acid extraction or by pepsin digestion. CNBr digestion (86%) of cementum and subsequent carboxymethyl cellulose chromatography suggests that human cementum consists of type I collagen only as identified by amino acid and hexose analyses.
用人牙获取牙骨质。通过盐和乙酸提取或胃蛋白酶消化,无法从牙骨质中显著溶解出胶原蛋白。对牙骨质进行溴化氰消化(86%)并随后进行羧甲基纤维素层析,结果表明,通过氨基酸和己糖分析鉴定,人牙骨质仅由I型胶原蛋白组成。