Jin K, Chen J, Kawaguchi K, Zhu R L, Stetler R A, Simon R P, Graham S H
Department of Neurology, University of Pittsburgh, School of Medicine, PA 15261, USA.
Neuroreport. 1996 Jul 29;7(11):1797-802. doi: 10.1097/00001756-199607290-00022.
GADD45 is a DNA damage-inducible gene that accelerates DNA excision repair and can be induced by a variety of DNA-damaging stimuli in mammalian cells. We investigated the expression of GADD45 mRNA and protein using in situ hybridization and immunocytochemistry in rat brains after 2 h of temporal focal ischemia. The expression of GADD45 mRNA was induced in neurons throughout ischemic cortex 4 h after the onset of ischemia but was restricted to ischemic penumbra regions at 24 h after ischemia. The expression of GADD45 protein was increased only in sublethally injured neurons in the penumbra regions at both 4 h and 24 h following ischemia. These results suggest that GADD45 could have a protective role in ischemic neurons.
GADD45是一种DNA损伤诱导基因，可加速DNA切除修复，并可被哺乳动物细胞中的多种DNA损伤刺激所诱导。我们使用原位杂交和免疫细胞化学方法，研究了短暂性局灶性脑缺血2小时后大鼠脑中GADD45 mRNA和蛋白的表达。缺血发作后4小时，整个缺血皮质的神经元中诱导了GADD45 mRNA的表达，但在缺血后24小时，其表达仅限于缺血半暗带区域。缺血后4小时和24小时，GADD45蛋白的表达仅在半暗带区域亚致死性损伤的神经元中增加。这些结果表明，GADD45可能对缺血神经元具有保护作用。
