Itoh T, Yonezawa S, Nomoto M, Ueno K, Kim Y S, Sato E
Second Department of Pathology, Faculty of Medicine, Kagoshima University, Japan.
Pathol Int. 1996 Sep;46(9):646-55. doi: 10.1111/j.1440-1827.1996.tb03667.x.
Recent studies have identified that mucin antigens and Lewis X (Lex)-related antigens behave like oncodevelopmental tumor-associated antigens in several human adenocarcinomas. However, the expression of these antigens in pharyngeal and laryngeal squamous cell carcinomas (SCC) and in the precursor lesion is not fully elucidated yet. In the present study, the expression of mucin core protein antigens associated with the MUC1 gene product (DF3 antigen, mammary-type apomucin) and the MUC2 gene product (intestinal-MRP antigen, intestinal-type apomucin) mucin carbohydrate antigens that are associated with the earliest steps in mucin glycosylation (Tn, sialyl-Tn and T), and Lex-related antigens (Lex, Ley and sialyl Lex-i) in biopsy or resected specimens from 26 normal squamous epithelia (NSE), 49 dysplastic squamous epithelia (DSE) and 51 SCC were examined. The DF3 antigen was not expressed in NSE (0%), whereas it was expressed in 20 DSE (41%) and in 31 SCC (61%). The intestinal-MRP antigen showed no expression in NSE, DSE or SCC. The Tn antigen showed no expression in NSE, but showed low expression rates in DSE (14%) and in SCC (16%). The sialyl-Tn and T antigens were expressed in NSE, as well as in DSE and SCC. The T antigen expression increased with progression from NSE to DSE to SCC, while the sialyl-Tn antigen did not show such a tendency. Any of the three Lex-related antigens showed no characteristic expression in DSE and SCC. In the eight antigens examined, only DF3 antigen was an effective marker for DSE and SCC in the pharyngeal and laryngeal region. Cytoplasmic expression of DF3 and sialyl-Tn antigens were more frequently seen in SCC than in DSE, and might be useful to differentiate SCC from DSE.
近期研究已证实，在几种人类腺癌中，黏蛋白抗原和Lewis X（Lex）相关抗原表现为肿瘤发生发展相关抗原。然而，这些抗原在咽喉部鳞状细胞癌（SCC）及其癌前病变中的表达情况尚未完全阐明。在本研究中，我们检测了26例正常鳞状上皮（NSE）、49例发育异常鳞状上皮（DSE）和51例SCC的活检或切除标本中，与MUC1基因产物相关的黏蛋白核心蛋白抗原（DF3抗原，乳腺型膜黏蛋白）、与MUC2基因产物相关的黏蛋白核心蛋白抗原（肠型MRP抗原，肠型膜黏蛋白）、与黏蛋白糖基化早期步骤相关的黏蛋白碳水化合物抗原（Tn、唾液酸Tn和T）以及Lex相关抗原（Lex、Ley和唾液酸Lex-i）的表达情况。DF3抗原在NSE中未表达（0%），而在20例DSE（41%）和31例SCC（61%）中表达。肠型MRP抗原在NSE、DSE或SCC中均未表达。Tn抗原在NSE中未表达，但在DSE（14%）和SCC（16%）中表达率较低。唾液酸Tn和T抗原在NSE、DSE和SCC中均有表达。T抗原的表达随病变从NSE进展至DSE再到SCC而增加，而唾液酸Tn抗原未表现出这种趋势。三种Lex相关抗原中的任何一种在DSE和SCC中均未表现出特征性表达。在所检测的八种抗原中，只有DF3抗原是咽喉部DSE和SCC的有效标志物。DF3和唾液酸Tn抗原的细胞质表达在SCC中比在DSE中更常见，可能有助于鉴别SCC和DSE。
