Kitamura H, Yonezawa S, Tanaka S, Kim Y S, Sato E
Second Department of Pathology, Faculty of Medicine, Kagoshima University, Kagoshima, Japan.
Jpn J Cancer Res. 1996 Jun;87(6):631-40. doi: 10.1111/j.1349-7006.1996.tb00270.x.
We examined the expression of carbohydrate antigens which are associated with the earliest steps in mucin glycosylation (Tn and sialosyl-Tn) and the expression of the mucin core protein antigens associated with MUC1 gene product (DF3 antigen) as well as MUC2 gene product (intestinal-MRP antigen) in tissues from 38 patients with carcinoma of the ampulla of Vater, in order to determine whether these mucin antigens are available as tumor markers or not, and to evaluate whether their expression is correlated with the biological behavior of the carcinomas or not. DF3 antigen showed a relatively high expression rate (61%) in the carcinoma tissues, but was rarely expressed in the non-neoplastic epithelium around the carcinomas in the region of the ampulla of Vater. Tn and sialosyl-Tn antigens showed high expression rates in the carcinoma tissues (86% and 84% each), whereas they showed rare or no expression in the non-neoplastic epithelium around the carcinomas, except for highly restricted expression in the duodenal villous epithelium. The patients with positive DF3 expression in the carcinoma showed significantly poorer survival than those with negative DF3 expression (P < 0.05), whereas the patients with positive intestinal-MRP expression in the carcinoma showed significantly more favorable survival than those with negative intestinal-MRP expression (P < 0.05). The expression rate of DF3 antigen was significantly higher in the cases with deep invasion into the pancreas (89%) than in those with no or minimal invasion (52%) (P < 0.05). In contrast, the expression rate of intestinal-MRP antigen was significantly higher in the cases with no or minimal invasion into the pancreas (38%) than in those with deep invasion (0%) (P<0.05). In conclusion, the expression of DF3, To and sialosyl-Tn antigens is an effective histopathological indicator for carcinomas in the area of the ampulla of Vater, and the expression of DF3 and intestinal-MRP antigens is a useful indicator of the prognosis of the patients.
我们检测了38例 Vater壶腹癌患者组织中与粘蛋白糖基化最早步骤相关的碳水化合物抗原（Tn和唾液酸化Tn）、与MUC1基因产物相关的粘蛋白核心蛋白抗原（DF3抗原）以及与MUC2基因产物相关的（肠-MRP抗原）的表达，以确定这些粘蛋白抗原是否可作为肿瘤标志物，并评估其表达是否与癌的生物学行为相关。DF3抗原在癌组织中显示出相对较高的表达率（61%），但在Vater壶腹区域癌周围的非肿瘤上皮中很少表达。Tn和唾液酸化Tn抗原在癌组织中显示出高表达率（各为86%和84%），而在癌周围的非肿瘤上皮中很少或无表达，十二指肠绒毛上皮有高度局限的表达除外。癌组织中DF3表达阳性的患者生存率明显低于DF3表达阴性的患者（P<0.05），而癌组织中肠-MRP表达阳性的患者生存率明显高于肠-MRP表达阴性的患者（P<0.05）。胰腺深度浸润的病例中DF3抗原的表达率（89%）明显高于无浸润或轻度浸润的病例（52%）（P<0.05）。相反，无浸润或轻度浸润胰腺的病例中肠-MRP抗原的表达率（38%）明显高于深度浸润的病例（0%）（P<0.05）。总之，DF3、Tn和唾液酸化Tn抗原的表达是Vater壶腹区域癌有效的组织病理学指标，DF3和肠-MRP抗原的表达是患者预后的有用指标。
