Lazo A, Bailer R T
Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus, USA.
J Med Primatol. 1996 Aug;25(4):257-66. doi: 10.1111/j.1600-0684.1996.tb00208.x.
Simian T-cell lymphotrophic virus type I (STLV-I) and human T-cell lymphotrophic virus types I/II (HTLV-I/II) contain the tax gene which can transactivate the transcription of viral and cellular genes including several cytokines. These investigations used two STLV-I and four different HTLV-I/II transformed cell lines to quantitate constitutive cytokine release, p24 antigen production, and to correlate gag p24 antigen and cytokine release. These investigations are the first to report 1) quantitative comparison of constitutive release of multiple cytokines by several STLV-I and HTLV-I/II transformed cell lines and to determine the cytokines constitutively released by STLV-I transformed cell lines as IL-6, b-FGF, and GM-CSF (and TNF-beta, and PDGF in a higher viral producing line); 2) statistically significant differences in levels of cytokines produced by STLV-I and HTLV-I/II transformed cell lines, dependent on the method of results quantification; and 3) a correlation between levels of STLV-I and HTLV-I/II and gag p24 antigen production and cytokine production.
猴免疫缺陷病毒I型（STLV-I）和人嗜T细胞病毒I/II型（HTLV-I/II）含有tax基因，该基因可反式激活病毒和细胞基因的转录，包括多种细胞因子。这些研究使用了两种STLV-I和四种不同的HTLV-I/II转化细胞系，以定量组成性细胞因子释放、p24抗原产生，并关联gag p24抗原与细胞因子释放。这些研究首次报告了：1）对几种STLV-I和HTLV-I/II转化细胞系组成性释放多种细胞因子进行定量比较，并确定STLV-I转化细胞系组成性释放的细胞因子为IL-6、b-FGF和GM-CSF（以及在高病毒产生系中的TNF-β和PDGF）；2）根据结果定量方法，STLV-I和HTLV-I/II转化细胞系产生的细胞因子水平存在统计学显著差异；3）STLV-I和HTLV-I/II水平与gag p24抗原产生及细胞因子产生之间的相关性。
