Kirby A R, Gunning A P, Morris V J
Institute of Food Research, Norwich Laboratory, UK.
Biopolymers. 1996 Mar;38(3):355-66. doi: 10.1002/(sici)1097-0282(199603)38:3<355::aid-bip8>3.0.co;2-t.
Techniques have been developed for the routine reliable imaging of polysaccharides by atomic force microscopy (AFM). The polysaccharides are deposited from aqueous solution onto the surface of freshly cleaved mica, air dried, and then imaged under alcohols. The rationale behind the development of the methodology is described and data is presented for the bacterial polysaccharides xanthan, acetan, and the plant polysaccharides l-carrageenan and pectin. Studies on uncoated polysaccharides have demonstrated the improved resolution achievable when compared to more traditional metal-coated samples or replicas. For acetan the present methodology has permitted imaging of the helical structure. Finally, in addition to data obtained on individual polysaccharides, AFM images have also been obtained of the network structures formed by kappa-carrageenan and gellan gum.
已经开发出通过原子力显微镜（AFM）对多糖进行常规可靠成像的技术。将多糖从水溶液沉积到新劈开的云母表面，空气干燥，然后在醇类环境下成像。描述了该方法开发背后的基本原理，并给出了细菌多糖黄原胶、乙酰聚糖以及植物多糖L-卡拉胶和果胶的数据。对未涂层多糖的研究表明，与更传统的金属涂层样品或复制品相比，该方法可实现更高的分辨率。对于乙酰聚糖，本方法能够对其螺旋结构进行成像。最后，除了获得的关于单个多糖的数据外，还获得了κ-卡拉胶和结冷胶形成的网络结构的AFM图像。
