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植物细胞壁、植物细胞壁多糖和凝胶的原子力显微镜观察

Atomic force microscopy of plant cell walls, plant cell wall polysaccharides and gels.

作者信息

Morris V J, Gunning A P, Kirby A R, Round A, Waldron K, Ng A

机构信息

Institute of Food Research, Norwich Research Park, Colney, UK.

出版信息

Int J Biol Macromol. 1997 Aug;21(1-2):61-6. doi: 10.1016/s0141-8130(97)00042-1.

DOI:10.1016/s0141-8130(97)00042-1
PMID:9283017
Abstract

Methods developed for the routine imaging of polysaccharides by atomic force microscopy (AFM) have been used to image plant polysaccharides from higher plants (pectin) and algae (carrageenan). These methods have been extended to image K-carrageenan association in hydrated films. Finally, AFM has been used to image polysaccharide architecture in moist plant cell walls. Simple experimental and image processing methods have been used to enhance molecular structure in 'rough' cell wall surfaces.

摘要

通过原子力显微镜(AFM)对多糖进行常规成像所开发的方法,已被用于对高等植物(果胶)和藻类(角叉菜胶)中的植物多糖进行成像。这些方法已扩展到对水合膜中κ-角叉菜胶的缔合进行成像。最后,AFM已被用于对潮湿植物细胞壁中的多糖结构进行成像。已使用简单的实验和图像处理方法来增强“粗糙”细胞壁表面的分子结构。

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