Buys C H, Ypma J M, Gouw W L
Hum Genet. 1979 Jun 19;49(2):129-32. doi: 10.1007/BF00277634.
In cultured amniotic fluid cells a mediocentric chromosome 9 appeared to be completely deficient in constitutive heterochromatin when stained with distamycin A and DAPI. In addition, this deficient chromosome was found in a blood cell culture from the father. Both the father and the child after birth were phenotypically normal. Evidently, a considerable heterozygotic deficit of chromosome 9 heterochromatin can be tolerated without affecting the phenotype. The heterochromatin defect was also shown by G11-staining. Distamycin A-DAPI staining is highly reproducible and is recommended as a fluorescent alternative to often less successful G11-methods for the detection of heteromorphism of chromosome 9.
在培养的羊水细胞中，用偏端霉素A和4',6-二脒基-2-苯基吲哚（DAPI）染色时，一条近中着丝粒9号染色体似乎完全缺乏组成型异染色质。此外，在父亲的血细胞培养物中也发现了这条缺失的染色体。父亲和出生后的孩子表型均正常。显然，9号染色体异染色质相当大的杂合性缺失可以被容忍而不影响表型。G11染色也显示了异染色质缺陷。偏端霉素A-DAPI染色具有高度可重复性，推荐作为一种荧光替代方法，用于检测9号染色体异态性，而G11方法往往不太成功。
