Avdienko V G, Kondrashov S Iu, Liashenko S M
Probl Tuberk. 1996(1):6-8.
Seven hybridomas of BALB/c mice producing monoclonal antibodies (mAb) against M. tuberculosis H37Rv have been obtained. The mAb acted against M. tuberculosis H36Rv with molecular mass 14, 17-15, 25 27 30 kD excluding mAb S5B3B8 and S3H5D7 which acted against the main antigen with 54 kD mass and 5-6 bands of antigens. Immunoglobulin isotypes of mAb were IgG2a and IgG2b. mAb can be divided into 2 groups by interaction with mycobacterial antigens in EIA: specific to M. tuberculosis complex antigens, cross-reactive to antigens of different mycobacteria. Chemical nature of antigenic determinants recognizable by a panel of mAb was investigated. Mild sodium periodate oxidation and protease digestion of mycobacterial antigens showed that mAb recognize both carbohydrate-containing epitopes (S2H6G4) and protein epitopes (S5B11E10, S5B3B8, S4C1G4) or protein and carbohydrate-containing antigenic determinants (S2F5C10, S2F2E2).
已获得7株BALB/c小鼠杂交瘤，它们产生针对结核分枝杆菌H37Rv的单克隆抗体（mAb）。这些单克隆抗体作用于结核分枝杆菌H36Rv，其分子量为14、17 - 15、25、27、30 kD，但不包括作用于54 kD主要抗原和5 - 6条抗原带的单克隆抗体S5B3B8和S3H5D7。单克隆抗体的免疫球蛋白亚型为IgG2a和IgG2b。通过酶免疫分析（EIA）中与分枝杆菌抗原的相互作用，单克隆抗体可分为2组：对结核分枝杆菌复合抗原特异，对不同分枝杆菌抗原交叉反应。研究了一组单克隆抗体可识别的抗原决定簇的化学性质。对分枝杆菌抗原进行温和的高碘酸钠氧化和蛋白酶消化表明，单克隆抗体既识别含碳水化合物的表位（S2H6G4），也识别蛋白质表位（S5B11E10、S5B3B8、S4C1G4）或含蛋白质和碳水化合物的抗原决定簇（S2F5C10、S2F2E2）。
