Csiba A, Szentgyörgyi M, Juhász S, Lombai G
Veterinary and Food Control Station of Capital Budapest, Hungary.
Acta Vet Hung. 1996;44(2):165-72.
A high-performance liquid chromatographic (HPLC) procedure with fluorometric detector has been developed for the determination of nitrite and formaldehyde from foods by the use of hydralazine. Hydralazine reacts with nitrite and formaldehyde under acidic conditions in boiling water-bath for 15 min to form tetrazolo-(5,1-a)-phthalazine (Tetra-P) and triazolo-(3,4-a)-phthalazine (Tri-P) quantitatively. Without extraction, the determination of Tetra-P and Tri-P was simple, specific, sensitive and reliable over the range of 0.003-0.3 ppm of sodium nitrite and 0.02-0.4 ppm of formaldehyde. This procedure using hydralazine is one of the most useful methods for routine analysis of nitrite and formaldehyde in foods, biological fluids and ambient waters.
已开发出一种带有荧光检测器的高效液相色谱（HPLC）方法，用于通过使用肼来测定食品中的亚硝酸盐和甲醛。肼在酸性条件下于沸水浴中与亚硝酸盐和甲醛反应15分钟，定量生成四唑并-(5,1-a)-酞嗪（Tetra-P）和三唑并-(3,4-a)-酞嗪（Tri-P）。无需萃取，在亚硝酸钠0.003 - 0.3 ppm和甲醛0.02 - 0.4 ppm范围内，对Tetra-P和Tri-P的测定简单、特异、灵敏且可靠。这种使用肼的方法是食品、生物体液和环境水中亚硝酸盐和甲醛常规分析最有用的方法之一。
