Riciputo R M, Oliveri S, Micali G, Sapuppo A
Institute of Microbiology, University of Catania, Italy.
Mycoses. 1996 May-Jun;39(5-6):233-5. doi: 10.1111/j.1439-0507.1996.tb00131.x.
The lipophilic dimorphic yeast Malassezia furfur is a common skin commensal and the aetiological agent of pityriasis versicolor. A source of lipids is essential for its growth, and there are already demonstrations of in vitro lipase and lipoxygenase production. In eight wild strains, isolated from patients with pityriasis versicolor, we showed a phospholipase activity using a medium containing egg yolk emulsion as the only source of lipids; in this medium M. furfur grows and produces a phospholipase zone. Adding manganese sulphate, an unspecific inhibitor of phospholipase activity, M. furfur does not grow, because the lipophilic fungus cannot utilize the egg yolk as a source of fatty acids. Adding Tween 60 to the same medium, M. furfur also grows in presence of manganese sulphate.
亲脂性双态酵母糠秕马拉色菌是一种常见的皮肤共生菌，也是花斑癣的病原体。脂质来源对其生长至关重要，并且已有体外产生脂肪酶和脂氧合酶的相关证明。在从花斑癣患者中分离出的8株野生菌株中，我们使用含有蛋黄乳液作为唯一脂质来源的培养基显示出磷脂酶活性；在这种培养基中，糠秕马拉色菌生长并产生磷脂酶区。添加磷脂酶活性的非特异性抑制剂硫酸锰后，糠秕马拉色菌无法生长，因为这种亲脂性真菌不能利用蛋黄作为脂肪酸来源。在相同培养基中添加吐温60，糠秕马拉色菌在硫酸锰存在的情况下也能生长。
