Mullock B M, Issa F S, Hinton R H
Clin Chim Acta. 1977 Aug 15;79(1):129-40. doi: 10.1016/0009-8981(77)90470-3.
Rat serum 5'-nucleotidase, L-leucyl-beta-naphthylamidase and beta-glycerophosphatase activities are increased whilst alkaline p-nitrophenylphosphatase and alkaline phosphodiesterase activities are unchanged or decreased three days after bile duct ligation. Affinity chromatography on an immobilised antiserum raised against highly purified liver plasma membranes showed that although 5'-nucleotidase in normal serum is unrelated to the 5'-nucleotidase of liver plasma membrane, the 5'-nucleotidase of bile and much of the 5'-nucleotidase in the jaundiced serum are closely related to the plasma membrane enzyme. Since bile is rich in 5'-nucleotidase, the changes in level of this enzyme after bile duct ligation are most simply explained by leakage of bile into the blood; changes in the patterns of the other enzymes are shown to be consistent with this explanation. The jaundiced serum was examined by gel exclusion chromatography and flotation in sucrose gradients for the presence of small fragments of plasma membrane as reported in human jaundiced sera, but no such fragments could be detected three days after bile-duct ligation.
胆管结扎三天后，大鼠血清5'-核苷酸酶、L-亮氨酰-β-萘酰胺酶和β-甘油磷酸酶活性升高，而碱性对硝基苯磷酸酶和碱性磷酸二酯酶活性未变或降低。用针对高度纯化的肝细胞膜制备的固定化抗血清进行亲和层析显示，尽管正常血清中的5'-核苷酸酶与肝细胞膜的5'-核苷酸酶无关，但胆汁中的5'-核苷酸酶以及黄疸血清中的大部分5'-核苷酸酶与质膜酶密切相关。由于胆汁富含5'-核苷酸酶，胆管结扎后该酶水平的变化最简单的解释是胆汁漏入血液；其他酶模式的变化也与这一解释一致。像在人类黄疸血清中报道的那样，通过凝胶排阻色谱法和蔗糖梯度漂浮法检测黄疸血清中是否存在质膜小片段，但胆管结扎三天后未检测到此类片段。
