Rabie A B, Lie Ken Jie R K
Department of Children's Dentistry and Orthodontics, Faculty of Dentistry, University of Hong Kong, Hong Kong.
Int J Oral Maxillofac Surg. 1996 Aug;25(4):311-8. doi: 10.1016/s0901-5027(06)80064-0.
The use of endochondral bone grafts (EC) and demineralized bone matrix (DBM), which contains a potent osteoinductive matrix, may promote the repair of nonregenerative defects. The purpose of the current work is to assess qualitatively and quantitatively the effect of DBM on the healing of EC bone grafts and to compare it to the healing of EC bone grafts alone. Twenty-four defects in rabbit skulls were filled with EC bone grafts alone, DBM alone, or combined EC and DBM. Histologic and immunohistologic changes were examined in 2 weeks. The amount of new bone formation was quantified by image analysis. Healing of all the groups was characterized by the presence of a cartilage intermediate stage. In the EC bone grafts alone, healing was localized to the host bone/graft interface. In the composite group, amalgamation of the new bone, DBM, and bone graft progressed throughout the whole width and depth of the defect, uniting the graft to the recipient bed. The amount of new bone formed was significantly greater (47%) in the composite group than the EC group. In conclusion, DBM powder augments the bone-induction capacity of the recipient bed as well as the bone graft. The composite EC bone grafts and added DBM possess properties required for an effective graft material and merit further clinical evaluation.
使用软骨内骨移植（EC）和含有强大骨诱导基质的脱矿骨基质（DBM），可能会促进非再生性缺损的修复。当前这项工作的目的是定性和定量评估DBM对EC骨移植愈合的影响，并将其与单纯EC骨移植的愈合情况进行比较。在兔颅骨上制造24处缺损，分别单独用EC骨移植、单独用DBM或联合使用EC和DBM进行填充。在2周时检查组织学和免疫组织学变化。通过图像分析对新骨形成量进行定量。所有组的愈合均以存在软骨中间阶段为特征。在单独的EC骨移植组中，愈合局限于宿主骨/移植界面。在复合组中，新骨、DBM和骨移植的融合在缺损的整个宽度和深度上进行，使移植与受区床结合。复合组形成的新骨量比EC组显著多（47%）。总之，DBM粉末增强了受区床以及骨移植的骨诱导能力。复合EC骨移植和添加的DBM具备有效移植材料所需的特性，值得进一步进行临床评估。
