Haig D M, Deane D L, Myatt N, Thomson J, Entrican G, Rothel J, Reid H W
Moredun Research Institute, Edinburgh, UK.
J Comp Pathol. 1996 Aug;115(2):163-74. doi: 10.1016/s0021-9975(96)80038-7.
The dynamics and activation status of CD4+ and CD8+ T-cells differentially expressing the CD45R (220 kDa) antigen were studied in prefemoral efferent lymph draining the site of cutaneous reinfection with orf virus. CD4+, CD45R+ lymphoblasts preceded CD4+, CD45R- lymphoblasts during the first 48 h after reinfection. Thereafter, the output of both total and blast-transformed CD4+, CD45R- T-cells increased in proportion to the CD4+, CD45R+ cells for the duration of the virus reinfection. Output of CD8+, CD45R+ T-cells exceeded that of the CD8+, CD45R+ cells both before and after reinfection. However, within the lymphoblast population, CD8+, CD45R+ and CD8+, CD45R- T-cells increased and decreased in parallel. CD4+, CD45R- and CD8+, CD45R- T-cells produced interleukin-2, interferon-gamma and granulocyte-macrophage colony-stimulating factor after culture for 24 h without exogenous restimulation, whereas CD4+, CD45R+ T-cells produced only interleukin-2. The results show that although both CD45R+ and CD45R- alpha beta receptor+ T-cell subsets are activated as a consequence of virus reinfection in vivo, it is the CD45R- subset that predominates in the later stages of reinfection and is the principal cellular source of lymphokines in the efferent lymph.
在引流皮肤感染羊口疮病毒部位的股前引流淋巴中，研究了差异表达CD45R（220 kDa）抗原的CD4 +和CD8 + T细胞的动力学及激活状态。再感染后的最初48小时内，CD4 +、CD45R +淋巴母细胞先于CD4 +、CD45R -淋巴母细胞出现。此后，在病毒再感染期间，总CD4 +、CD45R - T细胞和经有丝分裂原转化的CD4 +、CD45R - T细胞的输出量与CD4 +、CD45R +细胞成比例增加。再感染前后，CD8 +、CD45R + T细胞的输出量均超过CD8 +、CD45R -细胞。然而，在淋巴母细胞群体中，CD8 +、CD45R +和CD8 +、CD45R - T细胞呈平行增减。未经外源性再刺激培养24小时后, CD4 +、CD45R -和CD8 +、CD45R - T细胞产生白细胞介素-2、γ干扰素和粒细胞-巨噬细胞集落刺激因子，而CD4 +、CD45R + T细胞仅产生白细胞介素-2。结果表明，虽然CD45R +和CD45R -αβ受体+ T细胞亚群在体内病毒再感染后均被激活，但在再感染后期占主导地位的是CD45R -亚群，且它是引流淋巴中淋巴因子的主要细胞来源。
