Harki E, Klaebe A, Talou T, Dargent R
Laboratoire de Chimie Agro-industrielle, Ecole Nationale Supérieure de Chimie, Toulouse, France.
Steroids. 1996 Oct;61(10):609-12. doi: 10.1016/s0039-128x(96)00121-3.
The sterol composition of Tuber melanosporum was examined by medium-pressure liquid and high-performance liquid chromatography, nuclear magnetic resonance spectroscopy, and mass spectrometry. Ergosterol (ergosta-5,7,22-trienol) and brassicasterol (ergosta-5,22-dienol) were identified as the major components (90%). Their quantities and their relative concentrations, probably good indicators for differentiating mature truffles from immature colored ones, were determined during the maturation of the fungus. The results show that the total quantity of sterols decreased with the age of the fungus, but that the ergosterol/brassicasterol ratio remained relatively constant.
通过中压液相色谱、高效液相色谱、核磁共振光谱和质谱法对黑孢块菌的甾醇成分进行了检测。麦角甾醇（麦角甾-5,7,22-三烯醇）和油菜甾醇（麦角甾-5,22-二烯醇）被鉴定为主要成分（90%）。在真菌成熟过程中测定了它们的含量及其相对浓度，这可能是区分成熟松露与未成熟有色松露的良好指标。结果表明，甾醇总量随真菌年龄的增长而减少，但麦角甾醇/油菜甾醇的比例保持相对恒定。
