Antimicrobial efficacy of extracts of Saudi Arabian desert truffles.

BACKGROUND

truffles are known for their nutritional value and have been considered among traditional treatments for ophthalmic infections and ailments.

OBJECTIVES

We sought to investigate the antimicrobial efficacy of several extracts from Saudi Arabia. Certain pathogenic fungi and gram-negative and gram-positive bacteria were included.

METHODS

Dry extracts were prepared using methanol, ethyl acetate, and distilled water, while the latter was used for preparing fresh extracts. The extracts were microbiologically evaluated through the disc-diffusion agar method; the zones of inhibition of microbial growth were measured post-incubation. The minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) were determined in Müller-Hinton Broth through the microdilution susceptibility method. anti-biofilm activity was assessed for potent extracts.

RESULTS

Dry extracts showed potent activity (>16-mm inhibition zones) against gram-positive ( IFO3007 and IFO3060) and gram-negative ( IFO3448 and IFO3301) bacteria. The activity against fungi was moderate (12-16-mm inhibition zones) for both IFO4177 and IFO0583; there was no activity against IFO4414 growth. Methanolic extract had the lowest MIC and MBC, exhibiting remarkable activity against growth. Fresh extract showed moderate activity against bacterial growth and inactivity against fungal growth. Methanolic extract showed potent anti-biofilm activity (IC, 2.0 ± 0.18 mg/mL) against .

CONCLUSIONS

extracts showed antibacterial effects potentially suitable for clinical application, which warrants further in-depth analysis of their individual isolated compounds.