Palamara A T, Perno C F, Aquaro S, Buè M C, Dini L, Garaci E
Department of Experimental Medicine and Biochemical Sciences, University of Rome, Italy.
AIDS Res Hum Retroviruses. 1996 Nov 1;12(16):1537-41. doi: 10.1089/aid.1996.12.1537.
We investigated the effect of glutathione on the replication of human immunodeficiency virus (HIV) in chronically infected macrophages, a known reservoir of the virus in the body. We found that exogenous GSH strongly suppresses the production of p24gag protein as well as the virus infectivity. This is related to a dramatic decrease in both budding and release of virus particles from chronically infected cells (either macrophages or lymphocytes), together with a selective decrease in the expression of gp120, the major envelope glycoprotein, rich in intrachain disulfide bonds and thus potentially sensitive to the effect of a reducing agent such as GSH. Overall data suggest that GSH can interfere with late stages of virus replication. This would be in agreement with data obtained in cells exposed to herpesvirus type 1 (a DNA virus) or to Sendai (an RNA virus), showing that the suppression of virus replication by GSH is related to the selective inhibition of envelope glycoproteins. These results suggest a potential role of GSH in combination with other antivirals in the treatment of virus-related diseases.
我们研究了谷胱甘肽对慢性感染巨噬细胞中人类免疫缺陷病毒（HIV）复制的影响，慢性感染的巨噬细胞是体内已知的病毒储存库。我们发现，外源性谷胱甘肽强烈抑制p24gag蛋白的产生以及病毒的感染性。这与慢性感染细胞（巨噬细胞或淋巴细胞）中病毒颗粒的出芽和释放显著减少有关，同时富含链内二硫键且因此可能对诸如谷胱甘肽等还原剂的作用敏感的主要包膜糖蛋白gp120的表达选择性降低。总体数据表明，谷胱甘肽可干扰病毒复制的后期阶段。这与在暴露于1型疱疹病毒（一种DNA病毒）或仙台病毒（一种RNA病毒）的细胞中获得的数据一致，表明谷胱甘肽对病毒复制的抑制与包膜糖蛋白的选择性抑制有关。这些结果表明谷胱甘肽在与其他抗病毒药物联合治疗病毒相关疾病中具有潜在作用。
