Nobunaga T, Tokugawa Y, Hashimoto K, Kubota Y, Sawai K, Kimura T, Shimoya K, Takemura M, Matsuzaki N, Azuma C, Saji F
Department of Obstetrics and Gynecology, Osaka University, Medical School, Japan.
Am J Reprod Immunol. 1996 Oct;36(4):193-7. doi: 10.1111/j.1600-0897.1996.tb00162.x.
To evaluate the effect of nitric oxide in the seminal plasma on sperm motility.
Seminal plasma concentrations of NO2-, a stable end product of nitric oxide, of 108 males of infertile couples and 15 proven fertile donors were measured and compared with spermatogram parameters. Motile sperm was incubated with a nitric oxide-generating drug, sodium nitroprusside, for 6 hr in the absence or presence of oxyhemoglobin, an inhibitor of nitric oxide.
The NO2- concentration in the seminal plasma was 6.58 +/- 0.5.6 microM in 26 infertile males with leukocytospermia, 5.51 +/- 0.25 microM in 82 infertile males without leukocytospermia, and 3.91 +/- 0.16 microM in 15 controls. There was a significant correlation between the NO2- concentration and sperm motility. Sodium nitroprusside reduced the sperm motility in a dose- and time-dependent manner and its reduction was completely inhibited by the addition of oxyhemoglobin.
These findings indicate that nitric oxide concentration in the seminal plasma of infertile males is elevated and that nitric oxide is an inhibitor of sperm motion.
评估精浆中一氧化氮对精子活力的影响。
测定108对不育夫妇男性及15名已证实有生育能力的供者精浆中一氧化氮的稳定终产物NO2-的浓度，并与精液图参数进行比较。将活动精子与一氧化氮生成药物硝普钠一起孵育6小时，分别在有无一氧化氮抑制剂氧合血红蛋白存在的情况下进行。
26例白细胞精子症不育男性精浆中NO2-浓度为6.58±0.56微摩尔/升，82例无白细胞精子症不育男性为5.51±0.25微摩尔/升，15名对照者为3.91±0.16微摩尔/升。NO2-浓度与精子活力之间存在显著相关性。硝普钠以剂量和时间依赖性方式降低精子活力，加入氧合血红蛋白可完全抑制其降低作用。
这些发现表明不育男性精浆中一氧化氮浓度升高，且一氧化氮是精子运动的抑制剂。
