Herrero M B, Cebral E, Boquet M, Viggiano J M, Vitullo A, Gimeno M A
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET).
Acta Physiol Pharmacol Ther Latinoam. 1994;44(3):65-9.
Nitric oxide (NO) has recently been found to function as an intra and extra cellular messenger by activating guanylate cyclase. Its role in sperm hyperactivation was examined by adding to the capacitating medium a classical donor of NO (Sodium nitroprusside, NP) in two different concentrations (150 microM and 300 microM). In both treatments, the percentage of motile cells was evaluated, showing a significant decrease on motility and viability at 90 and 120 minutes when sodium nitroprusside was used in a concentration of 300 microM; no modifications were observed with 150 microM. The effect obtained with 300 microM of sodium nitroprusside was avoided by hemoglobin (20 micrograms/ml), a scavenger of the NO. The percentage of hyperactivated spermatozoa in the presence of 300 microM sodium nitroprusside increased significantly more than the control during the first 30 and 60 minutes of capacitation; but with 150 microM sodium nitroprusside a significant increase was observed at 60 and 90 minutes of incubation. Thus, the data strongly suggests that nitric oxide plays an important role in sperm hyperactivation "in vitro".
最近发现一氧化氮（NO）通过激活鸟苷酸环化酶作为细胞内和细胞外信使发挥作用。通过在获能培养基中添加两种不同浓度（150微摩尔和300微摩尔）的经典NO供体（硝普钠，NP）来研究其在精子超激活中的作用。在两种处理中，均评估了活动细胞的百分比，当使用300微摩尔浓度的硝普钠时，在90分钟和120分钟时活动力和活力均显著下降；而使用150微摩尔时未观察到变化。血红蛋白（20微克/毫升），一种NO清除剂，可避免300微摩尔硝普钠产生的影响。在获能的前30分钟和60分钟内，存在300微摩尔硝普钠时超激活精子的百分比显著高于对照组；但使用150微摩尔硝普钠时，在孵育60分钟和90分钟时观察到显著增加。因此，数据强烈表明一氧化氮在“体外”精子超激活中起重要作用。
