Mitsumori K, Takegawa K, Shimo T, Onodera H, Yasuhara K, Takahashi M
Division of Pathology, National Institute of Health Sciences, Tokyo, Japan.
Arch Toxicol. 1996;70(12):809-14. doi: 10.1007/s002040050343.
Changes observed in lympho-hematopoietic organs in rats given piperonyl butoxide may be attributable either to direct toxic effects or to undernutrition. Male F344 rats were therefore fed diet containing 2.5% piperonyl butoxide or subjected to a 64% restriction of food intake for 2 weeks. Marked inhibition of body weight gain, decreased white blood cell count, depletion of T/B lymphocytes in lymphoid tissues, hypoplasia of the bone marrow, and decreased proliferating cell nuclear antigen (PCNA) labeling indices in these tissues were seen in both dietary restriction and 2.5% piperonyl butoxide groups. The depletion of T lymphocytes in the thymus and spleen was stronger in the 2.5% piperonyl butoxide group, as indicated by PCNA labeling indices and image analysis of T lymphocyte areas of the spleen, however, the toxicological profile observed for the chemically treated group was essentially the same as for animals on the restricted diet. These results suggest that the lympho-hematopoietic findings in rats receiving 2.5% piperonyl butoxide are probably due to undernutrition resulting from a reduced food intake.
给予胡椒基丁醚的大鼠淋巴造血器官中观察到的变化，可能归因于直接毒性作用或营养不足。因此，对雄性F344大鼠喂食含2.5%胡椒基丁醚的饲料，或使其食物摄入量限制64%，持续2周。在饮食限制组和2.5%胡椒基丁醚组中，均可见体重增加明显受抑制、白细胞计数减少、淋巴组织中T/B淋巴细胞耗竭、骨髓发育不全以及这些组织中增殖细胞核抗原（PCNA）标记指数降低。根据PCNA标记指数以及脾脏T淋巴细胞区域的图像分析表明，2.5%胡椒基丁醚组胸腺和脾脏中T淋巴细胞的耗竭更为明显，然而，化学处理组观察到的毒理学特征与饮食受限动物基本相同。这些结果表明，摄入2.5%胡椒基丁醚的大鼠淋巴造血方面的表现可能是由于食物摄入量减少导致营养不足所致。
