Kasper M, Traub O, Reimann T, Bjermer L, Grossmann H, Müller M, Wenzel K W
Institute of Pathology, Technical University of Dresden, Germany.
Histochem Cell Biol. 1996 Oct;106(4):419-24. doi: 10.1007/BF02473301.
The degree of immunoreactive connexin43 (C x 43) in rat lung was evaluated during the development of radiation-induced pulmonary fibrosis in rat by a double immunofluorescence technique using polyclonal antisera to Cx43 and monoclonal antibodies to cytokeratins on cryostat sections. In normal rat lungs, Cx43 was detected in pneumocytes type II and I, in large blood vessel endothelia, in peribronchial smooth muscle cells, and in some peribronchial and perivascular interstitial cells. As early as 1 week after irradiation, enhanced immunoreactivity for Cx43 in the epithelial cells was detected. In severely injured lungs (about 3 months after irradiation), Cx43 was found also in the cytoplasm of type II pneumocytes. These findings were confirmed by western blot data. Western blot analysis also revealed increased phosphorylation of Cx43. It remains to be investigated whether the increased content of Cx43 in irradiated rat lung may be due to an enhanced number of gap junctions between type I and II alveolar epithelial cells.
利用抗连接蛋白43（Cx43）多克隆抗血清和抗细胞角蛋白单克隆抗体，采用双重免疫荧光技术，在低温恒温切片上评估大鼠放射性肺纤维化发展过程中大鼠肺组织中免疫反应性连接蛋白43（Cx43）的程度。在正常大鼠肺组织中，在Ⅱ型和Ⅰ型肺细胞、大血管内皮细胞、支气管周围平滑肌细胞以及一些支气管周围和血管周围间质细胞中检测到Cx43。照射后1周，即检测到上皮细胞中Cx43的免疫反应性增强。在严重受损的肺组织（照射后约3个月）中，也在Ⅱ型肺细胞的细胞质中发现了Cx43。这些发现得到了蛋白质免疫印迹数据的证实。蛋白质免疫印迹分析还显示Cx43的磷酸化增加。照射大鼠肺组织中Cx43含量增加是否可能是由于Ⅰ型和Ⅱ型肺泡上皮细胞之间缝隙连接数量增加，仍有待研究。
