Bonsignorio D, Perego L, Del Giacco L, Cotelli F
Department of Biology, University of Milan, Italy.
Zygote. 1996 May;4(2):101-8. doi: 10.1017/s0967199400002975.
The chorion is the acellular envelope surrounding mature eggs of teleostean fish. The macromolecular composition of the zebrafish (Danio rerio) egg chorion, organised as a three-layered structure, has been analysed. SDS-PAGE analysis, under reducing conditions, of isolated and purified chorions revealed a reproducible pattern of four major polypeptides (116, 97, 50 and 43 kDa) and several minor bands. Lectin binding assays showed that both the 116 kDa and 50 kDa proteins were recognised by concanavalin agglutinin (Con A), Galanthus nivalis agglutinin (GNA), Sambucus nigra bark agglutinin (SNA) and Ricinus communis agglutinin (RCA 120), suggesting that these polypeptides are N-linked glycoproteins. By contrast, neither the 97 kDa nor the 43 kDa polypeptides were stained by these lectins, indicating that these polypeptides are not glycosylated. Amino acid analysis also showed significant differences in the average content of some amino acids, for example serine and proline, when compared with previous reports.
绒毛膜是硬骨鱼成熟卵周围的无细胞包膜。斑马鱼（Danio rerio）卵绒毛膜的大分子组成呈三层结构，已被分析。在还原条件下，对分离和纯化的绒毛膜进行SDS-PAGE分析，揭示了四种主要多肽（116、97、50和43 kDa）和几条次要条带的可重复模式。凝集素结合试验表明，116 kDa和50 kDa的蛋白质均被伴刀豆球蛋白A（Con A）、雪花莲凝集素（GNA）、黑接骨木树皮凝集素（SNA）和蓖麻凝集素（RCA 120）识别，表明这些多肽是N-连接糖蛋白。相比之下，97 kDa和43 kDa的多肽均未被这些凝集素染色，表明这些多肽未被糖基化。氨基酸分析还显示，与之前的报告相比，某些氨基酸（如丝氨酸和脯氨酸）的平均含量存在显著差异。
