Hu L F, Eiriksdottir G, Lebedeva T, Kholodniouk I, Alimov A, Chen F, Luo Y, Zabarovsky E R, Ingvarsson S, Klein G, Ernberg I
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Genes Chromosomes Cancer. 1996 Oct;17(2):118-26. doi: 10.1002/(SICI)1098-2264(199610)17:2<118::AID-GCC7>3.0.CO;2-8.
We have examined 17 primary undifferentiated nasopharyngeal carcinoma biopsies for allelic loss on 3p, comparing the findings in tumors with those in normal lymphocyte DNA from the same patients. Ten polymorphic microsatellite markers were used between 3p13 and 3p26. Allelic loss was observed in 12 samples (70%). Two loci were most frequently affected: D3S1067 (3p21.1-14.3) in 60% and D3S1217 (3p14.2-14.1) in 58%. One tumor seemed to have a homozygous deletion at 3p26, detected by the D3S1297 marker. Analysis of the clinical data showed that an increased number of aberrations in 3p was correlated with more advanced tumor stages.
我们检测了17例原发性未分化鼻咽癌活检组织的3p等位基因缺失情况，将肿瘤中的检测结果与同一患者正常淋巴细胞DNA中的结果进行比较。在3p13和3p26之间使用了10个多态性微卫星标记。12个样本（70%）观察到等位基因缺失。两个位点受影响最频繁：60%的样本中D3S1067（3p21.1 - 14.3）和58%的样本中D3S1217（3p14.2 - 14.1）。通过D3S1297标记检测到一个肿瘤在3p26处似乎存在纯合缺失。临床数据分析表明，3p中异常数量增加与更晚期的肿瘤阶段相关。
