Deng L, Jing N, Tan G, Zhou M, Zhan F, Xie Y, Cao L, Li G
Cancer Research Institute and Xiang-Ya Hospital, Hunan Medical University, China.
Genes Chromosomes Cancer. 1998 Sep;23(1):21-5. doi: 10.1002/(sici)1098-2264(199809)23:1<21::aid-gcc4>3.0.co;2-8.
Loss of heterozygosity (LOH) of chromosome arm 3p has been commonly observed in carcinomas of various tissues, including those of nasopharyngeal carcinoma (NPC). To determine the frequency and extent of allelic loss in NPC, we investigated 16 loci on chromosome bands 3p21-26 in 24 tumor tissues by microsatellite analysis. LOH on 3p21-26 was found in 16 of 24 (66.7%) tumors. The highest frequency of allelic loss was found in two adjacent loci, D3S1620 (11/22, 50%) and D3S1560 (9/18, 50%). Eight cases showed LOH in one contiguous region and 5 cases in more than one region. Samples 1, 3, 4, 7, 8, 10, 16, 17, 18, 19, and 22 had a contiguous stretch of allelic loss between D3S1297 and D3S1597. The smallest common LOH/deletion region seems likely to lie between D3S1297 (3p26.3-26.2) and D3S1560 (3p25.3). The allelic loss map defined here will facilitate finer mapping of putative tumor suppressor gene loci and positional cloning of such genes, which may play a role in carcinogenesis of NPC.
在包括鼻咽癌（NPC）在内的各种组织的癌中，常观察到3号染色体短臂的杂合性缺失（LOH）。为了确定鼻咽癌中等位基因缺失的频率和范围，我们通过微卫星分析研究了24个肿瘤组织中3p21 - 26染色体带上的16个位点。在24个肿瘤中的16个（66.7%）发现了3p21 - 26上的LOH。等位基因缺失频率最高的是在两个相邻位点，D3S1620（11/22，50%）和D3S1560（9/18，50%）。8例在一个连续区域显示LOH，5例在一个以上区域显示。样本1、3、4、7、8、10、16、17、18、19和22在D3S1297和D3S1597之间有一段连续的等位基因缺失。最小的常见LOH/缺失区域似乎可能位于D3S1297（3p26.3 - 26.2）和D3S1560（3p25.3）之间。这里定义的等位基因缺失图谱将有助于更精细地定位假定的肿瘤抑制基因位点并对这些基因进行定位克隆，这些基因可能在鼻咽癌的致癌过程中起作用。
