cDNA和转录在酵母中诱导Ty重组：RAD1和RAD52基因的作用。
Induction of Ty recombination in yeast by cDNA and transcription: role of the RAD1 and RAD52 genes.
作者信息
Nevo-Caspi Y, Kupiec M
机构信息
Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat-Aviv, Israel.
出版信息
Genetics. 1996 Nov;144(3):947-55. doi: 10.1093/genetics/144.3.947.
Abstract
In the yeast Saccharomyces cerevisiae ectopic recombination has been shown to occur at high frequencies for artificially created repeats, but at relatively low frequencies for a natural family of repeated sequences, the Ty family. Little is known about the mechanism(s) that prevent recombination between repeated sequences. We have previously shown that nonreciprocal recombination (gene conversion) of a genetically marked Ty can be induced either by the presence of high levels of Ty cDNA or by transcription of the marked Ty from a GAL1 promoter. These two kinds of induction act in a synergistic manner. To further characterize these two kinds of Ty recombination, we have investigated the role played by the RAD52 and RAD1 genes. We have found that the RAD52 and RAD1 gene products are essential to carry out transcription-induced Ty conversion whereas cDNA-mediated conversion can take place in their absence.
摘要
在酿酒酵母中，异位重组已被证明在人工构建的重复序列中高频发生，但在一个天然的重复序列家族——Ty家族中发生频率相对较低。对于阻止重复序列之间重组的机制知之甚少。我们之前已经表明，高水平的Ty cDNA的存在或从GAL1启动子对标记的Ty进行转录，均可诱导带有遗传标记的Ty发生非相互重组（基因转换）。这两种诱导方式具有协同作用。为了进一步表征这两种Ty重组，我们研究了RAD52和RAD1基因所起的作用。我们发现，RAD52和RAD1基因产物对于进行转录诱导的Ty转换是必不可少的，而在它们缺失的情况下，cDNA介导的转换仍可发生。
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