Aramayo R, Peleg Y, Addison R, Metzenberg R
Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706, USA.
Genetics. 1996 Nov;144(3):991-1003. doi: 10.1093/genetics/144.3.991.
This report describes the identification, cloning, and molecular analysis of Asm-1+ (Ascospore maturation 1), the Neurospora crassa homologue of the Aspergillus nidulans stuA (stunted A) gene. The Asm-1+ gene is constitutively transcribed and encodes an abundant, nucleus-localized 68.5-kD protein. The protein product of Asm-1+ (ASM-1), contains a potential DNA-binding motif present in related proteins from A. nidulans (StuA), Candida albicans (EFGTF-1), and Saccharomyces cerevisiae (Phd1 and Sok2). This motif is related to the DNA binding motif of the Swi4/Mbp1/Res family of transcription factors that control the cell cycle. Deletion of Asm-1+ destroys the ability to make protoperithecia (female organs), but does not affect male-specific functions. We propose that the APSES domain (ASM-1, Phd1, StuA, EFGTF-1, and Sok2) defines a group of proteins that constitute a family of related transcription factors involved in the control of fungal development.
本报告描述了粗糙脉孢菌中构巢曲霉stuA（发育迟缓A）基因的同源基因Asm-1+（子囊孢子成熟1）的鉴定、克隆及分子分析。Asm-1+基因持续转录，编码一种丰富的、定位于细胞核的68.5-kD蛋白。Asm-1+（ASM-1）的蛋白产物含有一个潜在的DNA结合基序，该基序存在于构巢曲霉（StuA）、白色念珠菌（EFGTF-1）和酿酒酵母（Phd1和Sok2）的相关蛋白中。该基序与控制细胞周期的Swi4/Mbp1/Res转录因子家族的DNA结合基序相关。Asm-1+的缺失破坏了形成原雌器（雌性器官）的能力，但不影响雄性特异性功能。我们提出，APSES结构域（ASM-1、Phd1、StuA、EFGTF-1和Sok2）定义了一组蛋白，它们构成了一个参与真菌发育调控的相关转录因子家族。
