Karapetian A T, Mehrabian N M, Terzikian G A, Vardevanian P O, Antonian A P, Borisova O F, Frank-Kamenetskii M D
Yerevan Institute of Architecture and Construction, Armenia.
J Biomol Struct Dyn. 1996 Oct;14(2):275-83. doi: 10.1080/07391102.1996.10508118.
We treat theoretically conformational transitions in DNA-ligand complexes allowing for the existence of different binding parameters of the ligand to different DNA conformations. The parameters of binding are determined from the best fit of the theory to experimental data for the difference between transition point (Tm) and the width of transition curve (delta T) for the complexes and for naked DNA. The analysis shows that Ethidium Bromide (EB) and Actinomycin D (AMD) each may form at least five types of complexes: three types (one "strong" and two "weak") with helix DNA and two types ("strong" and "weak") with single-stranded DNA. The parameters of the complexes have been obtained. Some testable experimental predictions of the theory are also discussed.
我们从理论上探讨了DNA-配体复合物中的构象转变，同时考虑到配体与不同DNA构象存在不同的结合参数。结合参数通过将理论与复合物及裸露DNA的转变点（Tm）和转变曲线宽度（δT）差异的实验数据进行最佳拟合来确定。分析表明，溴化乙锭（EB）和放线菌素D（AMD）各自可能形成至少五种类型的复合物：三种类型（一种“强”和两种“弱”）与螺旋DNA结合，两种类型（“强”和“弱”）与单链DNA结合。已获得复合物的参数。还讨论了该理论的一些可检验的实验预测。
