Vidotto V, Bruatto M, Accattatis G, Caramello S
Dipartimento discipline Medico-Chirurgiche, sez. Malattie Infettive, Università di Torino, Italy.
New Microbiol. 1996 Oct;19(4):327-34.
The presence of red (RNA) and green (DNA) fluorescent material identifying nucleic acids in the chlamydospores of Candida albicans has been well documented. Red fluorescence in chlamydospores is relatively diffused and ranges from small spots, observed in hyphal cells, to the entire protoplasmic content. Green fluorescence is rarely visible in these structures and, when present it can be observed next to the plasmalemma. The initial percentage values of the two curves related to the cell counts of red fluorescence of the suspensor cells and chlamydospores showed remarkable differences between the two structures. About 54% of the chlamydospores showed red fluorescence compared to about 28% of the suspensor cells. It seems from the results obtained in this study that much RNA was produced and/or accumulated in the chlamydospores and suspensor cells, rather than in mycelium where red fluorescence was not observed. The results obtained sustain the hypothesis that a chlamydospore should he considered a fully functional cell that is morphologically and physiologically active and independent from mycelium.
在白色念珠菌厚垣孢子中存在可识别核酸的红色（RNA）和绿色（DNA）荧光物质，这已得到充分证明。厚垣孢子中的红色荧光相对弥散，范围从小斑点（在菌丝细胞中观察到）到整个原生质内容物。绿色荧光在这些结构中很少可见，当存在时，可在质膜附近观察到。与悬垂细胞和厚垣孢子红色荧光细胞计数相关的两条曲线的初始百分比值在这两种结构之间显示出显著差异。约54%的厚垣孢子显示红色荧光，而悬垂细胞约为28%。从本研究获得的结果来看，似乎在厚垣孢子和悬垂细胞中产生和/或积累了大量RNA，而在未观察到红色荧光的菌丝体中则没有。所获得的结果支持这样的假设，即厚垣孢子应被视为一个功能完全的细胞，在形态和生理上是活跃的，且独立于菌丝体。
