Olivieri F, Prasad V, Valbonesi P, Srivastava S, Ghosal-Chowdhury P, Barbieri L, Bolognesi A, Stirpe F
Dipartimento di Patologia Sperimentale dell'Università di Bologna, Italy.
FEBS Lett. 1996 Nov 4;396(2-3):132-4. doi: 10.1016/0014-5793(96)01089-7.
Two systemic antiviral resistance-inducing proteins, CIP-29 and CIP-34, isolated from Clerodendrum inerme Gaertn. leaves, were tested for ribosome-inactivating properties. It was found that CIP-29 has the characteristics of a polynucleotide:adenosine glycosidase (ribosome-inactivating protein), in that it inhibits protein synthesis both in cell-free systems and, at higher concentrations, in cells, and releases adenine from ribosomes, RNA, poly(A) and DNA. As compared with other known RIPs, CIP-29 deadenylates DNA at a high rate, and induces systemic antiviral resistance in susceptible plants.
从假茉莉叶片中分离出的两种系统性抗病毒抗性诱导蛋白CIP - 29和CIP - 34，对其核糖体失活特性进行了测试。结果发现，CIP - 29具有多核苷酸：腺苷糖苷酶（核糖体失活蛋白）的特性，因为它在无细胞系统中以及在较高浓度下在细胞中均能抑制蛋白质合成，并从核糖体、RNA、聚腺苷酸和DNA中释放腺嘌呤。与其他已知的核糖体失活蛋白相比，CIP - 29能高效地使DNA去腺苷酸化，并在易感植物中诱导系统性抗病毒抗性。
