Mirzoeva O K, Yaqoob P, Knox K A, Calder P C
A.N. Belozerzky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
FEBS Lett. 1996 Nov 4;396(2-3):266-70. doi: 10.1016/0014-5793(96)01111-8.
Two lipophilic derivatives of caffeic acid which inhibit lipoxygenase, caffeic acid phenethyl ester (CAPE) and N,N'-dicyclohexyl-O-(3,4-dihydroxycinnamoyl)-isourea (DCHCU), reduced the proliferative response of murine splenocytes to concanavalin A in vitro. Both CAPE and DCHCU induced apoptosis in murine thymocyte cultures as verified by flow cytometry and by visualisation of DNA with acridine orange staining. CAPE-induced apoptosis was inhibited by z-VAD-fmk, an inhibitor of the interleukin-1beta-converting enzyme family of cysteine proteases. We suggest that the lipoxygenase pathway of arachidonic acid metabolism plays a role in regulating lymphocyte responses such as proliferation and apoptosis.
两种抑制脂氧合酶的咖啡酸亲脂性衍生物，咖啡酸苯乙酯（CAPE）和N，N'-二环己基-O-（3,4-二羟基肉桂酰基）异脲（DCHCU），在体外降低了小鼠脾细胞对刀豆球蛋白A的增殖反应。通过流式细胞术和吖啶橙染色观察DNA证实，CAPE和DCHCU均可诱导小鼠胸腺细胞培养物中的细胞凋亡。半胱氨酸蛋白酶白细胞介素-1β转换酶家族的抑制剂z-VAD-fmk可抑制CAPE诱导的细胞凋亡。我们认为，花生四烯酸代谢的脂氧合酶途径在调节淋巴细胞反应如增殖和凋亡中起作用。
