Minor T, Yamaguchi T, Klauke H, Wingenfeld P, Michalk D, Isselhard W
Institute for Experimental Medicine, University of Cologne, Germany.
Adv Exp Med Biol. 1996;403:157-61. doi: 10.1007/978-1-4899-0182-8_18.
Livers of male Wistar rats (250-300 g) were isolated and flushed with 10 ml of Ringer's solution and 10 ml of UW preservation solution. Then the organs were stored for 24 h at 4 degrees C in UW solution. Livers of Group 1 were rinsed with 10 ml of Ringer's solution and reperfused after hypothermic storage with oxygenated Krebs-Henseleit solution (95% O2; 5% CO2) in a nonrecirculating system at constant pressure (10 mmHg) and 37 degrees C. Livers of Group 2 were incubated for 30 min at 37 degrees C prior to reperfusion, in order to simulate rewarming of the organ upon surgical implantation. Livers of Group 3 were treated like Group 2, but taurine was admixed to the UW solution (1 mM). Livers of Group 1 showed little signs of a preservation/reperfusion injury, with low enzyme activities of the parenchymal ALT and endothelial purine nucleoside phosphorylase (PNP) in the postischemic rinse solution (ALT: 19.9 +/- 12.4; PNP: 3.3 +/- 0.4 U/liter), adequate portal flow values about 3 ml/g/min and high O2 uptake at the end of the experiment (VO2: 3.2 +/- 0.4 ml/100g/min). Livers of Group 2 exhibited nearly tenfold higher enzyme activities in the rinse solution (ALT: 247.0 +/- 94.7*; PNP: 29.5 +/- 17.0* U/l) and disturbed tissue perfusion with significantly reduced flow values of about 2 ml/g/min during the first 10 min of reperfusion. As a result, the recovery of O2 uptake was only 2.2 +/- 0.3 ml/100 g/min*. Addition of taurine (Group 3) resulted in a significant reduction of the enzyme loss (ALT: 96.2 +/- 50.0#; PNP:12.4 +/- 7.0# U/liter) and improved portal flow values and O2 uptake at the end of reperfusion (2.7 +/- 0.3 ml/100 g/min#). The results give evidence for the importance of the rewarming period after hypothermic storage, which is inevitable during implantation of the organ in vivo. Taurine seems to exert a protective effect, affecting both the vascular endothelium and parenchymal tissue (*p < 0.05 vs Group 1; # p < 0.05 vs Group 2).
选取体重250 - 300克的雄性Wistar大鼠，分离其肝脏，先用10毫升林格氏液和10毫升UW保存液冲洗。然后将器官在UW溶液中于4℃保存24小时。第1组肝脏先用10毫升林格氏液冲洗，低温保存后，在非循环系统中于37℃、恒压（10 mmHg）下用充氧的克氏 - 亨氏液（95% O₂；5% CO₂）进行再灌注。第2组肝脏在再灌注前于37℃孵育30分钟，以模拟器官在手术植入时的复温过程。第3组肝脏的处理方式与第2组相同，但在UW溶液中加入了牛磺酸（1 mM）。第1组肝脏几乎没有保存/再灌注损伤的迹象，缺血后冲洗液中实质细胞谷丙转氨酶（ALT）和内皮嘌呤核苷磷酸化酶（PNP）的酶活性较低（ALT：19.9±12.4；PNP：3.3±0.4 U/升），门静脉血流值充足，约为3毫升/克/分钟，实验结束时氧摄取量较高（VO₂：3.2±0.4毫升/100克/分钟）。第2组肝脏冲洗液中的酶活性几乎高出10倍（ALT：247.0±94.7*；PNP：29.5±17.0* U/升），组织灌注受到干扰，再灌注最初10分钟内血流值显著降低，约为2毫升/克/分钟。结果，氧摄取量的恢复仅为2.2±0.3毫升/100克/分钟*。添加牛磺酸（第3组）导致酶损失显著减少（ALT：96.2±50.0#；PNP：12.4±7.0# U/升），并改善了门静脉血流值，再灌注结束时氧摄取量增加（2.7±0.3毫升/100克/分钟#）。这些结果证明了低温保存后复温期的重要性，这在体内器官植入过程中是不可避免的。牛磺酸似乎发挥了保护作用，对血管内皮和实质组织均有影响（*与第1组相比，p < 0.05；#与第2组相比，p < 0.05）。
