Yang J L, Ow K T, Russell P J, Ham J M, Crowe P J
Department of Surgery, Prince of Wales Hospital, University of New South Wales, Sydney, Australia.
Ann Surg Oncol. 1996 Nov;3(6):574-9. doi: 10.1007/BF02306092.
Expression of individual oncogenes may predict outcome in patients with metastatic colorectal cancer (CRC). We studied the oncogene profile in the tumors of patients with CRC and assessed their value as predictors of liver metastases.
The oncoproteins c-myc, c-erbB-2/neu (c-neu), PCNA and p53, were measured by immunohistochemistry in sections of metastasizing human CRC (n = 34) and their liver secondaries as well as in sections of nonmetastasizing human CRC (n = 25).
The metastasizing primary CRC expressed proliferating-cell nuclear antigen (PCNA), c-neu, and c-myc at significantly higher levels than the nonmetastasizing primary cancer, p53 was also overexpressed in the metastatic group compared with the nonmetastasizing CRC, but this difference was not significant. The frequency of expression of all these markers was similar in the metastasizing primary CRC and the liver secondaries from the same patients. There was no correlation between the expression of the individual markers and histological grade, DNA ploidy, and subsequent local recurrence and lung metastasis and survival. However, when both groups were assessed together, positive expression of c-myc was more likely to occur in poorly differentiated tumors, whereas PCNA expression increased with more advanced Dukes stages.
These results suggest that the overexpression of c-myc, c-neu, PCNA, and p53 may occur in CRC that are likely to metastasis to the liver.
个别癌基因的表达可能预测转移性结直肠癌（CRC）患者的预后。我们研究了CRC患者肿瘤中的癌基因谱，并评估了它们作为肝转移预测指标的价值。
通过免疫组织化学法检测转移性人类CRC（n = 34）及其肝转移灶以及非转移性人类CRC（n = 25）切片中的癌蛋白c-myc、c-erbB-2/neu（c-neu）、增殖细胞核抗原（PCNA）和p53。
转移性原发性CRC中增殖细胞核抗原（PCNA）、c-neu和c-myc的表达水平显著高于非转移性原发性癌。与非转移性CRC相比，p53在转移组中也有过表达，但差异不显著。在转移性原发性CRC和同一患者的肝转移灶中，所有这些标志物的表达频率相似。单个标志物的表达与组织学分级、DNA倍体、随后的局部复发、肺转移及生存率之间均无相关性。然而，当对两组一起评估时，c-myc的阳性表达更可能出现在低分化肿瘤中，而PCNA的表达随Dukes分期的进展而增加。
这些结果表明，c-myc、c-neu、PCNA和p53的过表达可能发生在可能转移至肝脏的CRC中。
