Sobreviela T, Pagcatipunan M, Kroin J S, Mufson E J
Department of Neurological Sciences, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois, USA.
J Comp Neurol. 1996 Nov 18;375(3):417-44. doi: 10.1002/(SICI)1096-9861(19961118)375:3<417::AID-CNE6>3.0.CO;2-5.
Brain-derived neurotrophic factor (BDNF) was the second member of the nerve growth factor (NGF) family to be isolated. The ability of BDNF to be retrogradely transported following intraparenchymal infusion represents a unique neurobiological tool to determine the location of putative neuron-specific BDNF-responsive neuronal systems. In the present study, we infused recombinant human (rh) BDNF into the rodent neo- and limbic cortex and used a turkey anti-BDNF antibody to determine specific populations of neurons which retrogradely transport this neurotrophin. Frontal cortex infusion retrogradely labeled neurons within the ipsilateral and contralateral frontal cortex, basal forebrain, lateral hypothalamus, centrolateral, mediodorsal, ventrolateral, ventromedial, ventral posterior, rhomboid, reuniens, and medial geniculate thalamic nuclei, and locus coeruleus. Occipital cortex infusion retrogradely labeled neurons in the frontal, temporal, occipital, and perirhinal cortices as well as the claustrum, basal forebrain, thalamus, epithalamus, hypothalamus, and raphe nuclei. Dorsal hippocampal infusion retrogradely labeled neurons within the septal diagonal band, supramammillary nucleus, and entorhinal cortex and was also transported within various hippocampal subfields. Entorhinal cortex infusion retrogradely labeled neurons within the perirhinal cortex, endopiriform nucleus, piriform cortex, dentate gyrus, presubiculum, parasubiculum, CA1-CA4 fields, amygdaloid nuclei, basal forebrain, thalamus, hypothalamus, periaqueductal gray, raphe nuclei, and locus coeruleus. Amygdala infusion labeled neurons in the endopiriform nucleus, temporal cortex, piriform cortex, paralimbic cortex, hippocampus, subiculum, entorhinal cortex, amygdala, basal forebrain, thalamus, hypothalamus, substantia nigra, pars compacta, raphe, and pontine parabrachial nuclei. In situ hybridization experiments demonstrated that virtually all areas which retrogradely transport BDNF also express its message. Neuroanatomical distributional studies of BDNF will unravel specific central nervous system neurotrophic-responsive systems.
脑源性神经营养因子（BDNF）是神经生长因子（NGF）家族中第二个被分离出来的成员。脑实质内注入BDNF后其逆行运输的能力，代表了一种独特的神经生物学工具，可用于确定假定的神经元特异性BDNF反应性神经元系统的位置。在本研究中，我们将重组人（rh）BDNF注入啮齿动物的新皮质和边缘皮质，并使用抗BDNF火鸡抗体来确定逆行运输这种神经营养因子的特定神经元群体。额叶皮质注入后，同侧和对侧额叶皮质、基底前脑、外侧下丘脑、中央外侧核、背内侧核、腹外侧核、腹内侧核、腹后核、菱形核、连合核和内侧膝状体丘脑核以及蓝斑中的神经元被逆行标记。枕叶皮质注入后，额叶、颞叶、枕叶和嗅周皮质以及屏状核、基底前脑、丘脑、上丘脑、下丘脑和中缝核中的神经元被逆行标记。背侧海马注入后，隔区斜角带、乳头体上核和内嗅皮质中的神经元被逆行标记，并且也在海马的各个亚区中运输。内嗅皮质注入后，嗅周皮质、内梨状核、梨状皮质、齿状回、前下托、下托、CA1 - CA4区、杏仁核、基底前脑、丘脑、下丘脑、导水管周围灰质、中缝核和蓝斑中的神经元被逆行标记。杏仁核注入后，内梨状核、颞叶皮质、梨状皮质、边缘旁皮质、海马、下托、内嗅皮质、杏仁核、基底前脑、丘脑、下丘脑、黑质致密部、中缝核和脑桥臂旁核中的神经元被标记。原位杂交实验表明，几乎所有逆行运输BDNF的区域也表达其信使核糖核酸。BDNF的神经解剖分布研究将揭示特定的中枢神经系统神经营养反应系统。
