大鼠脑不同区域多巴胺代谢的动力学

The dynamics of dopamine metabolism in various regions of rat brain.

作者信息

Karoum F, Neff N H, Wyatt R J

出版信息

Eur J Pharmacol. 1977 Aug 15;44(4):311-8. doi: 10.1016/0014-2999(77)90304-1.

Abstract

Dopamine metabolism was studied in various regions of rat brain by following the decline of 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) from brain after treatment with pargyline, or from the accumulation of the acids after treatment with probenecid. The decline of DOPAC and HVA after pargyline treatment appeared exponential in all regions of brain studied with half-lives of about 13 min for HVA and 6.5 min for DOPAC. DOPAC was the major metabolite of dopamine, with various brain regions producing between 2-5 times more DOPAC than HVA. HVA accumulated after treatment with probenecid but the accumulation in 1 h did ot account for all of the HVA apparently eliminated from brain. DOPAC accumulated in some regions of brain (medulla, hypothalamus and midbrain) and not in others (cerebellum, cortex, striatum and hippocampus) after probenecid treatment. We conclude that dopamine metabolism is not uniform in brain and that the accumulation of DOPAC and HVA in brain after probenecid treatment only accounts for a minor fraction of the dopamine formed in brain.

摘要

通过观察在用优降宁处理后大脑中3,4-二羟基苯乙酸（DOPAC）和3-甲氧基-4-羟基苯乙酸（HVA）的减少情况，或在用丙磺舒处理后这些酸的积累情况，研究了大鼠大脑不同区域的多巴胺代谢。在用优降宁处理后，DOPAC和HVA在所有研究的脑区中的减少呈指数形式，HVA的半衰期约为13分钟，DOPAC的半衰期约为6.5分钟。DOPAC是多巴胺的主要代谢产物，不同脑区产生的DOPAC比HVA多2至5倍。在用丙磺舒处理后HVA会积累，但1小时内的积累量并未占明显从大脑中消除的所有HVA。在用丙磺舒处理后，DOPAC在大脑的某些区域（延髓、下丘脑和中脑）积累，而在其他区域（小脑、皮层、纹状体和海马体）则不积累。我们得出结论，大脑中的多巴胺代谢并不均匀，并且在用丙磺舒处理后大脑中DOPAC和HVA的积累仅占大脑中形成的多巴胺的一小部分。